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Method for removing USEPA PAHs in agricultural soil by using aboriginal PAHs degradation bacterial community

A technology for degrading bacteria and communities, which is applied to the methods of using microorganisms, the restoration of contaminated soil, and methods based on microorganisms. It can solve the problems of large amounts of microorganisms, undiscovered, unfavorable natural soil microbial population balance, etc.

Active Publication Date: 2019-09-17
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, only one or some purely cultured microorganisms act on polluted soil, although there is a certain degradation effect, not only the amount of microorganisms is large, but also it is not conducive to the balance of natural soil microbial populations
At present, no method has been found to use domesticated and enriched indigenous PAHs-degrading bacteria to simultaneously remove polycyclic aromatic hydrocarbons (USEPA PAHs), which are prioritized by the US Environmental Protection Agency, in farmland soils

Method used

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  • Method for removing USEPA PAHs in agricultural soil by using aboriginal PAHs degradation bacterial community
  • Method for removing USEPA PAHs in agricultural soil by using aboriginal PAHs degradation bacterial community
  • Method for removing USEPA PAHs in agricultural soil by using aboriginal PAHs degradation bacterial community

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Embodiment Construction

[0039] The present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0040] 1. Preparation of indigenous PAHs degrading bacterial community:

[0041] (1) Preparation of soil leachate: Mix uncontaminated soil and water at a ratio of 1:10 and stir evenly. After standing for 2 hours, centrifuge the supernatant at 5000 rpm / min for 10 minutes to remove insoluble impurities, and sterilize at 121°C. Store at room temperature after 20 min.

[0042] (2) First-class seed solution: inoculate the suspension of polluted soil into the soil leach solution according to the inoculation amount of 10%, and then add the final concentration of 30mg L -1 The 10 kinds of PAHs were domesticated and enriched after 16 days. Sampling once every two days, part of the culture solution was centrifuged at 5000rpm / min to extract its genomic DNA, and the other part was added with glycerol with a final conce...

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Abstract

The invention discloses a method for removing USEPA PAHs in agricultural soil by using an aboriginal PAHs degradation bacterial community. The method comprises the steps of domesticizing and enriching autochthonous bacteria in soil by using PAHs as the only carbon source, monitoring the abundance of polycyclic aromatic hydrocarbon dioxygenase alpha subunit (RHD alpha) and the bacterial community changes in a culture solution, sorting appropriate bacterial community, performing extended culture, and performing inoculating in soil to reduce PADs content in the soil. The aboriginal PAHs degradation bacterial community has high-content RHD alpha genes. According to the method, USEPA PAHS in polluted farmland can be effectively removed by increasing the abundance of the RHD alpha gene in nidA and nahAc in agricultural soil, and the method has the advantages of being high in efficiency and environment-friendly.

Description

technical field [0001] The invention relates to the technical field of environmental engineering microorganisms, in particular to a method for removing USEPA PAHs in farmland soil by using indigenous PAHs-degrading bacterial communities. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of refractory and high-risk organic pollutants that widely exist in the environment. It has been found that there are as many as several hundred kinds of known PAHs. Due to their "three causes" effects of carcinogenicity, teratogenicity, and mutagenicity, they have the characteristics of wide distribution, large concealment, and difficult treatment in soil. Among them, 16 PAHs are listed as priority control PAHs by the US Environmental Protection Agency. Due to sewage irrigation, atmospheric deposition, and petrochemical leakage, soil PAHs pollution in my country and the world is becoming more and more serious. According to the National Soil Pollution Survey ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20B09C1/10C12R1/025C12R1/38C12R1/41C12R1/01
CPCB09C1/10C12N1/20C12N1/36
Inventor 高彦征陆超刘娟
Owner NANJING AGRICULTURAL UNIVERSITY
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