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Bivalent freeze-dried egg yolk antibody for infectious feline rhinitis-conjunctivitis and feline panleukopenia and preparation and application thereof

A technology for leukopenia and rhinoconjunctivitis, applied in the direction of antibodies, extracellular fluid diseases, immunoglobulin, etc., can solve the problems of lack of high specificity, low vaccine penetration rate, easy to produce drug resistance, etc., and achieve stability Good performance, low cost, high cost effect

Inactive Publication Date: 2019-09-17
CHANGCHUN SR BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the popularization rate of the vaccine is very low. Once infected, there is no specific treatment at home and abroad.
[0005] At present, broad-spectrum antibiotics are mainly used to prevent secondary infection and symptomatic treatment after cats are sick. However, antibiotics can interfere with protein synthesis by destroying cell walls. Long-term use will cause flora imbalance, and they are not highly specific and prone to drug resistance. The effect is not significant

Method used

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  • Bivalent freeze-dried egg yolk antibody for infectious feline rhinitis-conjunctivitis and feline panleukopenia and preparation and application thereof
  • Bivalent freeze-dried egg yolk antibody for infectious feline rhinitis-conjunctivitis and feline panleukopenia and preparation and application thereof
  • Bivalent freeze-dried egg yolk antibody for infectious feline rhinitis-conjunctivitis and feline panleukopenia and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 feline infectious rhinoconjunctivitis and feline panleukopenia dual vaccine

[0039] Utilize the insect cell-baculovirus expression system to produce feline infectious rhinoconjunctivitis and feline panleukopenia virus-like particles (using the method whose publication number is CN 108371710A), mix the two kinds of virus-like particles, and use β-propane After ester inactivation, add preservatives and adjuvants to prepare a double genetic engineering vaccine, and before mixing, the content of feline calicivirus virus-like particles is not less than 4mg / ml, and the hemagglutination titer of feline panleukopenia virus is not less than 1:2 14 .

Embodiment 2

[0040] Example 2 Screening of laying hens

[0041] The layer hens were selected and divided into two groups, namely the Hailan white layer group and the Hailan brown layer group. Use the double genetic engineering vaccine prepared in Example 1 to immunize two groups of laying hens respectively for 3 times, and the immunization doses are 1ml / only, 1.5ml / only, 2ml / only, and the immunization interval is 10 days, and the leg muscles are divided. For injection, each injection point should not exceed 1ml. Collect two groups of eggs 15 days after the third immunization, randomly select 10 eggs from each group, separate the egg white and egg yolk, dilute the egg yolk 10 times with pH 5.0 acetic acid-sodium acetate buffer, overnight, centrifuge at 4500r / min for 15 minutes, and take the supernatant , and the pH of the supernatant was adjusted to 6.5-7.0. The yolk antibody was extracted, and the neutralizing antibody titer of feline infectious rhinoconjunctivitis virus was determined b...

Embodiment 3 2

[0045] Example 3 Preparation of double freeze-dried egg yolk antibody

[0046] After sterilizing the high-free eggs collected in Example 2, use a separator to separate the egg white and egg yolk, dilute the egg yolk with pH5.0 acetic acid-sodium acetate buffer solution 10 times the volume of the egg yolk, stir until the egg yolk is completely dissolved, and let stand at 2-8°C Settled for 12 hours.

[0047] The first ammonium sulfate precipitation: Centrifuge the 10-fold acid dilution solution at 4500r / min for 15 minutes, and take the supernatant. The supernatant was purified by precipitation for the first time with a final concentration of 33% solid ammonium sulfate, stirred until the ammonium sulfate was completely dissolved, and allowed to stand at 2-8° C. for 2 hours for precipitation.

[0048] The second ammonium sulfate precipitation: centrifuge the first ammonium sulfate precipitation solution at 4500r / min for 15 minutes, and take the precipitate. The precipitate was d...

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Abstract

The invention discloses a bivalent freeze-dried egg yolk antibody for infectious feline rhinitis-conjunctivitis and feline panleukopenia and a preparation method and an application thereof, and belongs to the technical field of veterinary biopharmaceuticals. The bivalent freeze-dried egg yolk antibody for the infectious feline rhinitis-conjunctivitis and the feline panleukopenia can be directly prepared by bivalent vaccine immunized laying hens without hurting animals, and is simple in process method and low in cost; and the obtained bivalent antibody is high in titer, good in stability, quick to take effect, free of producing drug resistance, capable of being used for rapid treatment or emergency prevention of the infectious feline rhinitis-conjunctivitis and the feline panleukopenia and high in protection rate. Compared with vaccines and antibiotics, the invention has wider and more practical application prospects.

Description

technical field [0001] The invention relates to the technical field of veterinary biopharmaceuticals, in particular to a dual freeze-dried egg yolk antibody for cat infectious rhinoconjunctivitis and cat panleukopenia and its preparation and application. Background technique [0002] Feline infectious rhinoconjunctivitis is a viral respiratory disease caused by feline calicivirus (FCV). inflammation. The disease is highly contagious and mainly affects kittens under 1 year old. Feline infectious rhinoconjunctivitis infection alone will not be fatal, but the immunity of cats after infection will be weakened, and it is very prone to mixed infection with other feline upper respiratory viruses, which will lead to increased mortality. [0003] Feline panleukopenia, also known as feline infectious enteritis and feline distemper, is an acute, highly contagious infectious disease caused by feline panleukopenia virus (FPV). The main symptoms are sudden high fever, intractable vomit...

Claims

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Application Information

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IPC IPC(8): C07K16/02C07K16/10C07K16/08A61K39/395A61P27/02A61P7/00
CPCA61K2039/505A61P7/00A61P27/02C07K16/02C07K16/081C07K16/10C07K2317/11
Inventor 张馨月石晶夏振强殷玉和付玉陈宪平崔玉梅陶志
Owner CHANGCHUN SR BIOLOGICAL TECH
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