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Metal nanoparticles with peroxidase activity and preparation method thereof

A technology of metal nanoparticles and peroxidase, which is applied in metal processing equipment, nanotechnology, transportation and packaging, etc., to achieve the effect of short cycle, simple method and mild conditions

Inactive Publication Date: 2019-09-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the synthesis of metal nanoparticles with peroxidase activity by using reduced coenzyme

Method used

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  • Metal nanoparticles with peroxidase activity and preparation method thereof
  • Metal nanoparticles with peroxidase activity and preparation method thereof
  • Metal nanoparticles with peroxidase activity and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A preparation method of palladium nanoparticles with peroxidase activity, the specific steps are as follows:

[0032] (1) Add 100 μL of 40 mM sodium dihydrogen phosphate-disodium hydrogen phosphate with a pH of 5.0 and 20 μL of 100 mM sodium tetrachloropalladate aqueous solution into a 1.5 mL centrifuge tube;

[0033] (2) Add 40 μL of 100 mM reduced coenzyme I disodium salt solution to the liquid obtained in step (1), add distilled water to make the final volume 400 μL, shake and mix, and stand at 25°C for 12 hours to obtain Enzyme-active palladium nanoparticle liquid (named NADH-Pd2.0 liquid), obtained by transmission electron microscope scanning, has an average particle diameter of 1.7nm (see figure 1 ).

[0034] Determination of Peroxidase Activity of Palladium Nanoparticles:

[0035] Experiment 1: Take 12 μL of the palladium nanoparticles liquid with peroxidase activity obtained in Example 1 in a 500 μL centrifuge tube and add 188 μL of distilled water for dilutio...

Embodiment 2

[0043] A preparation method of palladium nanoparticles with peroxidase activity, the specific steps are as follows:

[0044] (1) Add 200 μL of 20 mM potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a pH of 5.0 and 25 μL of 80 mM sodium tetrachloropalladate aqueous solution into a 1.5 mL centrifuge tube;

[0045] (2) Add 20 μL of 20 mM reduced coenzyme I disodium salt solution to the liquid obtained in step (1), add distilled water to make the final volume 400 μL, shake and mix, and stand at 20°C for 24 hours to obtain The enzyme-active palladium nanoparticle liquid (named NADH-Pd0.2 liquid) has an average particle diameter of 1.1 nm obtained by scanning a transmission electron microscope.

[0046] Determination method is the same as embodiment 1, to the K of 3,3',5,5'-tetramethylbenzidine m The value is 0.019mM, the K of hydrogen peroxide m The value is 142.4 mM.

[0047] Replace the sodium tetrachloropalladate of this embodiment with potas...

Embodiment 3

[0051] A preparation method of palladium nanoparticles with peroxidase activity, the specific steps are as follows:

[0052] (1) Add 100 μL of 40 mM sodium dihydrogen phosphate-phosphate buffer solution with a pH of 5.0 and 100 μL of 20 mM sodium tetrachloropalladate solution into a 1.5 mL centrifuge tube;

[0053] (2) Add 100 μL of 10 mM reduced coenzyme I disodium salt solution to the liquid obtained in step (1), add distilled water to make the final volume 400 μL, oscillate and mix, and stand at 25°C for 24 hours to obtain The active palladium nanoparticle liquid (named as NADH-Pd0.5 liquid) has an average particle diameter of 1.3 nm obtained by scanning a transmission electron microscope.

[0054] Determination method is the same as embodiment 1, to the K of 3,3',5,5'-tetramethylbenzidine m The value is 0.031mM, the K of hydrogen peroxide m The value is 100.3 mM.

[0055] Replace the sodium tetrachloropalladate of this embodiment with potassium tetrachloroplatinate, rep...

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Abstract

The invention discloses metal nanoparticles with peroxidase activity and a preparation method thereof. The preparation method comprises the following steps of (1), 50-200 microliters of phosphate buffered solution at 20-80 mM and pH value 5.0 and 20-100 micro liters of sodium tetrachloropalladate aqueous solution at 20-100 mM are added to a centrifuge tube of 1.5 mL; and (2), 20-100 microliters of reduced coenzyme sodium salt solution at 10-100 mM is added to the liquid obtained in the step (1), distilled water is added to make the final volume of the liquid be 400 microliters, the liquid is stirred and mixed evenly, and the liquid mixture is left to stand at 20-30 DEG C for 8-24 hours to obtain the metal nanoparticle liquid with peroxidase activity. The method is simple, short in period, mild in conditions, and easy to control, and facilitates large-scale industrial production and preparation; and the metal nanoparticles with peroxidase activity have high catalytic activity.

Description

technical field [0001] The invention belongs to the technical field of nanoparticle preparation, and in particular relates to metal nanoparticle with peroxidase activity and a preparation method. Background technique [0002] Nanoparticles have attracted extensive attention due to their small size and large specific surface area. Metal nanoparticles, as an important part of nanoparticles, have important applications in the fields of energy, petrochemical, biocatalysis, and medicine. For example, gold nanoparticles have been widely used in medicine, disease diagnosis and drug delivery systems; nano-silver has been used in sensor technology, biomedicine and other fields; platinum and palladium nanoparticles have been widely used in the fields of sensing, catalysis and electrocatalysis [ACS SustainableChem.Eng.2013,1,591-602], due to the synergistic effect of two metals with different properties, bimetallic nanoparticles can enhance their catalytic activity, selectivity and sta...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00B82Y40/00
CPCB22F9/24B82Y40/00B22F2009/245B22F1/054
Inventor 付雁郑珊珊李韡张金利王桂倩
Owner TIANJIN UNIV
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