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Lactic acid bacteria powder for relieving heat stress of broilers and application thereof

A technology of heat stress and lactic acid bacteria, applied in the field of microecological preparations for livestock and poultry feeding, and lactic acid bacteria powder, can solve the problem of heat stress broiler production performance, blood indicators and intestinal morphology that have not been reported in the literature by Lactobacillus plantarum P-8 And immune function and other problems, to prevent toxins from invading the body, increase the number of viable bacteria, and improve production performance

Inactive Publication Date: 2019-09-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through a comprehensive search and analysis of the existing technologies, it is found that there is no literature reporting that Lactobacillus plantarum P-8 can improve the heat stress response by regulating the intestinal health of heat-stressed broilers, and there is no literature reporting that Lactobacillus plantarum P-8 has effects on Heat stress broiler performance, blood parameters and intestinal morphology and immune function have ideal regulation

Method used

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  • Lactic acid bacteria powder for relieving heat stress of broilers and application thereof
  • Lactic acid bacteria powder for relieving heat stress of broilers and application thereof
  • Lactic acid bacteria powder for relieving heat stress of broilers and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of Lactobacillus plantarum P-8 microecological bacteria powder

[0024] (1) Centrifuge the fermentation broth of Lactobacillus plantarum P-8 at 4000r / min for 20min at 4°C, centrifuge the obtained sludge, wash twice with distilled water, then add 2% (w / w) sorbitol solution to prepare Bacterial suspension, the number of viable bacteria is 2.0×10 12 CFU / mL, spare;

[0025](2) Add the sterilized kaolin into the bacterial suspension according to the mass percentage of 3% (w / w), stir, fully absorb the bacteria for 5 hours, and then pre-cool at -20°C for 12 hours, and then pre-cool the The frozen bacterial agent was put into a vacuum freeze dryer to dry, the freezing temperature was -55°C, the vacuum degree was 5mtorr, and the time was 26h. After the freeze drying was completed, the Lactobacillus plantarum P-8 microecological bacterial powder was obtained. After testing, the number of live bacteria of Lactobacillus plantarum P-8 after rehydratio...

Embodiment 2

[0026] Embodiment 2: the preparation of Lactobacillus plantarum P-8 microecological bacteria powder

[0027] (1) Centrifuge the fermentation broth of Lactobacillus plantarum P-8 at 4000r / min for 20min at 4°C, centrifuge the obtained sludge, wash twice with distilled water, then add 3% (w / w) sorbitol solution to prepare Bacterial suspension, the number of viable bacteria is 2.0×10 12 CFU / mL, spare;

[0028] (2) Add the sterilized kaolin into the bacterial suspension according to the mass percentage of 3.5% (w / w), stir, fully absorb the bacteria for 5 hours, and then pre-cool at -20°C for 12 hours, and then pre-cool the The frozen bacterial agent was put into a vacuum freeze dryer to dry, the freezing temperature was -55°C, the vacuum degree was 5mtorr, and the time was 26h. After the freeze drying was completed, the Lactobacillus plantarum P-8 microecological bacterial powder was obtained. After testing, the number of live bacteria of Lactobacillus plantarum P-8 after rehydra...

Embodiment 3

[0029] Embodiment 3: the preparation of Lactobacillus plantarum P-8 microecological bacteria powder

[0030] (1) Centrifuge the fermentation broth of Lactobacillus plantarum P-8 at 4000r / min for 20min at 4°C, centrifuge the obtained sludge, wash twice with distilled water, then add 3% (w / w) mannitol solution to prepare bacteria Suspension, the number of viable bacteria is 2.0×10 12 CFU / mL, spare;

[0031] (2) Add the sterilized kaolin into the bacterial suspension according to the mass percentage of 3.5% (w / w), stir, fully absorb the bacteria for 5 hours, and then pre-cool at -20°C for 12 hours, and then pre-cool the The frozen bacterial agent was put into a vacuum freeze dryer to dry, the freezing temperature was -55°C, the vacuum degree was 5mtorr, and the time was 26h. After the freeze drying was completed, the Lactobacillus plantarum P-8 microecological bacterial powder was obtained. After testing, the number of live bacteria of Lactobacillus plantarum P-8 after rehydrat...

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Abstract

The invention provides a lactic acid bacteria powder for relieving heat stress of broilers and an application thereof. The bacteria powder is obtained by adsorbing, embedding and lyophilizing a Lactobacillus plantarum P-8 bacteria solution through kaolin clay, and the lactobacillus plantarum P-8 bacteria liquid contains a lyophilized protective agent sorbitol. The lactobacillus plantarum P-8 can increase the jejunal villus height and velvet ratio of heat-stressed broilers, reduces the secretion of proinflammatory cytokines such as interleukin-1 and alpha-tumor necrosis, and achieves rapid restoration on intestinal mucosal damage in heat-stressed broilers, the repaired intestinal epithelial tissue not only facilitates the absorption of nutrients, but also prevents the toxin from invading the body, and the product effectively solves the problem of heat stress in broilers and improves the performance of broilers under high temperature conditions.

Description

technical field [0001] The invention belongs to the field of micro-ecological preparations, in particular relates to a micro-ecological preparation for raising livestock and poultry, in particular to a lactic acid bacteria powder for relieving broiler heat stress and its application. Background technique [0002] Broiler chickens are heat-sensitive animals, and heat stress is a strong stress for broiler chickens, and it is one of the important causes of chicken stress. It is generally believed that 10-32°C is the normal environment for chickens to maintain normal body temperature. 21-26°C is the comfortable environment temperature range for chickens to survive, and broiler chickens can exert the best growth performance. 26-32°C is the temperature range for chickens that are not comfortable but can maintain normal physiological functions. Physiological dysfunction can reduce the performance of broilers and can have a serious negative impact on the health of broilers and incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/163A23K50/75
CPCA23K10/18A23K20/163A23K50/75
Inventor 刘华伟赵金山李珂吕孝国
Owner QINGDAO AGRI UNIV
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