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Biocontrol bacterium Simplicillium lamellicola JC-1 and application thereof

A technology of cctccno., plant pathogenic bacteria, applied in the field of agricultural microbiology, to achieve good disease prevention effect, good inhibition effect, and avoid adverse effects

Active Publication Date: 2019-08-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, Simplicillium fungi mainly include 9 species including S.lamellicola, S.aogashimaense, S.lanosoniveum, S.chinense, S.obclavatum, S.cylindrosporum, S.subtropicum, S.minatense and S.sympodiophorum. Relevant studies on activity and metabolites are rarely reported at home and abroad

Method used

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  • Biocontrol bacterium Simplicillium lamellicola JC-1 and application thereof
  • Biocontrol bacterium Simplicillium lamellicola JC-1 and application thereof
  • Biocontrol bacterium Simplicillium lamellicola JC-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation, identification and biological characteristics analysis of Simplicillium lamellicola JC-1

[0026] 1. Isolation of strains and analysis of their biological characteristics

[0027] The applicant collected saprophytic rapeseed siliques from Chibi City, Hubei Province, cut the tissue block at the junction of disease and health on the saprophytic siliques, and the size was about 0.5cm×0.5cm; treated with 75% ethanol and 5% sodium hypochlorite for 1 minute respectively , and then washed with sterile water for 3 consecutive times, each time for 3 seconds. Then it is transferred to the center of a PDA medium plate containing 25% lactic acid (containing 80 μL of lactic acid solution (25%). After the plant tissue is dried on the plate, it is cultivated at a constant temperature of 20°C for 4 days, and finally it will grow out from the edge of the diseased tissue. The fungus was purified to obtain a fungus named JC-1.

[0028] 2. Morphological characteristics of stra...

Embodiment 2

[0038] Purification and Fermentation of Simplicillium lamellicola JC-1

[0039](1) Strain activation: Pick a piece of the slant of the JC-1 strain stored in a 4°C refrigerator with a sterile inoculation needle, inoculate it on PDA medium, and culture it in a 20°C incubator for 7 days.

[0040] (2) Strain cultivation: the JC-1 strain cultured for 7 days in step (1) was transferred to fresh PDA medium, and cultured in a 20° C. incubator for 7 days.

[0041] (3) Fermentation: Use a sterile puncher (diameter 6mm) to make some bacteria cakes on the edge of the JC-1 bacterial colony in step (2), inoculate 5 mycelium pieces in every 100mL MCD culture solution, 150rpm, 20 Cultivate with shaking for 3 days, 6 days, 9 days and 15 days respectively, and collect the filtrates of the fermented products of different days (filtering with 4 layers of gauze is enough) for use in the following examples.

[0042] Described MCD substratum is: 40g glucose, 2g L-alanine, 1g KH 2 PO 4 ·3H 2 O, 0...

Embodiment 3

[0044] Antagonistic Effects of Simplicillium lamellicola JC-1 on Four Important Rapeseed Pathogenic Bacteria

[0045] In this experiment, 4 kinds of pathogenic bacteria of important rapeseed diseases (black shank of rapeseed, Botrytis cinerea, Sclerotinia and Rhizoctonia solani) were selected for confrontation culture with JC-1 strain to determine the antibacterial activity of JC-1 strain.

[0046] Because the growth rate of JC-1 bacterial strain is slow, therefore, inoculate JC-1 bacterial strain (on PDA culture medium 15 days) in advance on plate, each plate inoculates a piece of JC-1 bacterium cake (diameter 6mm), wait for JC-1 1 When the colony diameter reaches 2cm, inoculate Botrytis cinerea, Sclerotinia and Rhizoctonia solani at 5cm from the edge of the JC-1 mycelium block. The inoculum of these bacteria was obtained from the edge of fresh colonies cultured for 2 days, and the rapeseed blackleg was taken from the edge of fresh colonies cultured for 7 days. The diameter o...

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Abstract

The invention belongs to the technical field of agricultural microbiology, and discloses a biocontrol bacterium Simplicillium lamellicola JC-1 and an application thereof. The preservation number of the biocontrol bacterium Simplicillium lamellicola JC-1 is CCTCC NO:M2019246. A Simplicillium lamellicola strain or fermentation substance filtrate thereof can effectively restrain growth of mycelia ofimportant pathogenic bacteria (sclerotinia scleroterum, botrytis cinerea, rhizoctonia solani and leptosphaeria biglobosa) on rape. Compared with chemically-synthesized pesticides which are used for control at present, the biocontrol bacterium Simplicillium lamellicola JC-1 has the advantages of being efficient, low in toxicity, free from environment pollution, and the like, and drug resistance isnot liable to cause because that the biocontrol bacterium Simplicillium lamellicola JC-1 is from natural environment, and the biocontrol bacterium Simplicillium lamellicola JC-1 has great applicationprospects.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbiology, and in particular relates to a biocontrol bacterium Simplicillium lamellicola JC-1 and its application. Background technique [0002] Simplicillium belongs to Ascomycota, Pezizomycotina, Sordariormycetes, Hypocreomycetidae, Hypocreales, Cordyceps family (Cordycipitaceae). In the early 21st century, Zare et al. established a new genus, Simplicillium, after reclassifying some species of Cephalosporium, Acremonium, and Verticillium. There are not many reports about Simplicillium at home and abroad, even less in China. Simplicillium has not been given a definite Chinese name by any authoritative organization in China. Duan Aili et al. called Simplicillium Paecilomyces in a report. Currently, Simplicillium fungi mainly include 9 species including S.lamellicola, S.aogashimaense, S.lanosoniveum, S.chinense, S.obclavatum, S.cylindrosporum, S.subtropicum, S.minatense and S.sympodiopho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCC12R2001/645C12N1/145A01N63/30
Inventor 李国庆李俊成张静杨龙吴明德
Owner HUAZHONG AGRI UNIV
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