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Preparation method of rabbit-derived swine fever heat-resistant protective agent live vaccine

A heat-resistant protective agent and live vaccine technology, applied in biochemical equipment and methods, vaccines, veterinary vaccines, etc., to achieve the effects of high content of active ingredients, pure vaccines, and improved solubility

Inactive Publication Date: 2019-08-30
山西隆克尔生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects of the existing technology, and add a new process in the process, that is, to remove the fat residue twice, so that the preparation method of the live vaccine of the heat-resistant protective agent for swine fever is more scientific, and the solubility of the vaccine is improved. Exogenous virus detection in rabbits does not result in product failure due to fat embolism causing rabbit death

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of a rabbit-derived heat-resistant protective agent live vaccine for swine fever, comprising the following steps: 1) Tissue mashing: put 2000 g of spleen drenched tissue with ice ballast into a mashing tank, add breast milk liquid, and start mashing machine to mash for 25min, after the mashing is completed, the emulsion is taken out, the spleen and lymphoid tissues of 18024 batches are mixed in a ratio of 1:1, and the volume ratio of the spleen and lymphoid tissues to the diluent is 1:5 ; 2) Filtration: Filter the mashed emulsion into a glass bottle through a double-layer 80-mesh stainless steel mesh to obtain tissue fluid, and collect the residue to weigh; 3) Centrifugation: Centrifuge the filtered tissue fluid at 4000r / min for 5min, control temperature below 10°C; 4) secondary filtration: after centrifugation, the tissue fluid is filtered with a single layer of 80-mesh stainless steel mesh, and the residue is collected and weighed; 5) mixing: the t...

Embodiment 2

[0026] A kind of preparation method of live vaccine of heat-resistant protective agent of rabbit-derived swine fever, comprising the following steps: 1) Tissue mashing: put 2000 g of splenic drench tissue with ice ballast shape into a mashing tank, add normal saline, and start the mashing machine Mashing was carried out for 35 minutes, and the emulsion was taken out after the mashing was completed. The spleen lymphoid tissue was mixed with 18025 batches of spleen and lymphoid tissue in a ratio of 1:1, and the volume ratio of the spleen lymphoid tissue to the diluent was 1:4; 2) Filtration: filter the crushed emulsion through a double-layer 80-mesh stainless steel mesh into a glass bottle to obtain tissue fluid, and collect the residue to weigh; 3) Centrifugation: centrifuge the filtered tissue fluid at 2800r / min for 15min, and control the temperature Below 10°C; 4) Secondary filtration: After centrifugation, the interstitial fluid is filtered with a single layer of 80-mesh stai...

Embodiment 3

[0028] A kind of preparation method of live vaccine of heat-resistant protective agent of rabbit-derived swine fever, comprising the following steps: 1) Tissue mashing: 2000 g of spleen drench tissue with ice ballast shape is packed into a mashing tank, PBS liquid is added, and the mashing machine is started Mashing was carried out for 30 minutes, and the emulsion was taken out after the mashing was completed. The spleen lymphoid tissue was 18026 batches of spleen and lymphoid tissue mixed in a ratio of 1:1, and the volume ratio of the spleen lymphoid tissue to the diluent was 1:5; 2) Filtration: filter the crushed emulsion through a double-layer 80-mesh stainless steel mesh into a glass bottle to obtain tissue fluid, and collect the residue and weigh it; 3) Centrifugation: Centrifuge the filtered tissue fluid at 3000r / min for 10min and control the temperature Below 10°C; 4) Secondary filtration: After centrifugation, the interstitial fluid is filtered with a single layer of 80...

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Abstract

The invention discloses a preparation method of rabbit-derived swine fever heat-resistant protective agent live vaccine. The preparation method comprises the following steps: 1) mashing a tissue: putting a spleen lymphoid tissue into a mashing cylinder, adding diluent, starting a mashing machine to perform mashing, and taking out an emulsifier after mashing; 2) filtering: filtering the mashed emulsifier into a glass bottle through a stainless steel net to obtain tissue fluid; 3) centrifuging: centrifuging the tissue fluid after filtering at 2800 to 4000 r / min for 5 to 15 minutes under the condition of controlling the temperature to be 10 DEG C or below; 4) performing secondary filtering: filtering the tissue fluid by using the stainless steel net after centrifuging; 5) mixing: adding a protective agent into the tissue fluid after secondary filtering; and 6) subpackaging and freeze-drying. Excessive residues and fat in the tissue fluie can be removed by a method of mashing, filtering, centrifuging and filtering. Thereby, the vaccine is purer and the effective component content is higher.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and in particular relates to a preparation method of a live vaccine of a heat-resistant protective agent for swine fever from rabbits. Background technique [0002] Swine fever is a kind of acute heat contact infectious disease caused by swine fever virus. It is characterized by septic pathological changes, visceral hemorrhage, infarction and necrosis. Swine fever is one of the important infectious diseases that threaten the pig industry. It can occur all year round and often causes devastating losses to the pig industry. The most effective way to prevent swine fever is to vaccinate against swine fever. There are mainly two kinds of swine fever vaccines: live swine fever vaccine (cell source) and live vaccine of swine fever heat-resistant protective agent (rabbit source). Among them, if the fat of the heat-resistant protective agent live vaccine for hog fever (rabbit source) is not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/26A61K47/34A61K47/42A61K39/187A61P31/14
CPCA61K9/19A61K39/12A61K47/26A61K47/34A61K47/42A61K2039/5254A61K2039/552A61P31/14C12N2770/24334
Inventor 宋宝敏张立峰曹晓剑徐明伟胡兆国
Owner 山西隆克尔生物制药有限公司
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