PCDR (polymerase chain displacement reaction) detection reagent for food-borne pathogenic bacterium listeria monocytogenes

Abstract

The invention relates to a PCDR (polymerase chain displacement reaction) detection reagent for food-borne pathogenic bacterium listeria monocytogenes, which can effectively realize quick and accuratedetection of the food-borne pathogenic bacterium listeria monocytogenes by a high-sensitive PCDR method. The PCDR detection reagent is prepared by an SD buffer solution, dNTPs, Mg<2+>, one pair of upstream and downstream inner primers, one pair of upstream and downstream outer primers and SD DNA polymerase through uniformly mixing by adding ultrapure water; when being used, the genomic DNA of thelisteria monocytogenes is dissolved in ddH2O, the DNA and the detection reagent provided by the invention are taken to be mixed and are uniformly mixed in an EP tube; the reaction tube is placed in aPCR (polymerase chain reaction) amplifier for reaction, predegeneration, annealing and extending, the steps are cycled, then agarose gel electrophoresis detection is performed, thereby realizing the detection of the food-borne pathogenic bacterium listeria monocytogenes by the PCDR method. The detection reagent provided by the invention is scientific and reasonable in components, easy to produce and prepare, convenient to use, and can quickly detect the food-borne pathogenic bacterium listeria monocytogenes with sensitivity and specificity, so as to ensure the food safety and realize obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a reagent for detecting food-borne pathogenic bacteria Listeria monocytogenes by PCDR method. Background technique [0002] With the vigorous development of the food industry, the types and quantities of food are increasing, especially in recent years, frequent food safety incidents caused by food-borne pathogens have sounded the alarm for people time and time again, and it is more and more urgent to Efficient, rapid and accurate detection of sexual pathogenic bacteria. The traditional detection method of foodborne pathogen Listeria monocytogenes (i.e. Listeria monocytogenes) involves pre-enrichment, selective isolation and culture, biochemical experiments and serological identification. cumbersome, low specificity and many other shortcomings. Therefore, the country has always attached great importance to the research on the detection of food-borne pathogens, hoping to find a detecti...

AI Technical Summary

Problems solved by technology

The present invention mainly studies the detection of Listeria monocytogenes by the PCDR method, designs two pairs of nested primers for the target gene, and explores the reaction system, and provides a food-borne pathogenic bacteria Listeria monocytogenes PCDR method detection Reagents and detection methods are given, but no public reports have been seen so far

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