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Colorimetric determination method for perchlorate in food through dispersive liquid-liquid microextraction-smart phone

A technology of dispersion liquid and smart phone, which is applied in the direction of analyzing materials through chemical reactions and material analysis through observing the influence of chemical indicators, and can solve the problems of strong equipment specificity, high operating costs, and market share. Low-level problems, to achieve the effect of low price, high sensitivity and good accuracy

Inactive Publication Date: 2019-08-16
酒泉市食品检验检测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mass spectrometry can accurately measure samples with very demanding content, but high operating costs, strong equipment specificity, low market share, and high requirements for laboratory hardware and personnel quality cannot achieve on-site testing

Method used

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  • Colorimetric determination method for perchlorate in food through dispersive liquid-liquid microextraction-smart phone
  • Colorimetric determination method for perchlorate in food through dispersive liquid-liquid microextraction-smart phone
  • Colorimetric determination method for perchlorate in food through dispersive liquid-liquid microextraction-smart phone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the mensuration of perchlorate in drinking water and bottled natural drinking mineral water

[0032] Sample preparation: direct assay.

[0033] Determination of samples: add 8mL sample, 550μL methylene blue solution (50μg / L), 1mL acetic acid-sodium acetate buffer solution with pH=4.5 to a 15mL centrifuge tube in turn, dilute to 10mL, shake well, let stand for 2min, add 70μL Methanol, 350μL of dichloromethane, vortex extraction for 60s, centrifugation at 4000r / min for 5min, the organic phase of the enriched sample was obtained at the bottom of the centrifuge tube; after standing for 30min, use the Android mobile software Color Grab to take pictures of the organic phase at the bottom of the centrifuge tube to obtain the organic phase RGB Color value; the color relative intensity value I of the organic phase of the enriched sample is brought into the linear regression equation of the standard curve to obtain the ClO in the sample purification solution 4 - C...

Embodiment 2

[0034] The mensuration of perchlorate in embodiment 2, tea beverage

[0035] Preparation of samples: Take 40mL of tea beverage in a 50mL centrifuge tube, add 40μL of glacial acetic acid, vortex to mix, and ultrasonic for 10min. 18 The solid phase extraction column was purified and passed through a 0.22 μm filter membrane.

[0036] Determination of samples: add 8mL sample, 550μL methylene blue solution (50μg / L), 1mL acetic acid-sodium acetate buffer solution with pH=4.5 to a 15mL centrifuge tube in turn, dilute to 10mL, shake well, let stand for 2min, add 70μL Methanol, 350μL of dichloromethane, vortex extraction for 60s, centrifugation at 4000r / min for 5min, the organic phase of the enriched sample was obtained at the bottom of the centrifuge tube; after standing for 30min, use the Android mobile software Color Grab to take pictures of the organic phase at the bottom of the centrifuge tube to obtain the organic phase RGB Color value; the color relative intensity value I of th...

Embodiment 3

[0037] The mensuration of perchlorate in embodiment 3, tealeaves, dehydrated spinach

[0038] Preparation of tea leaves and dehydrated spinach samples: crush tea leaves and dehydrated spinach and pass through a 100-mesh sieve, weigh 0.25~3g of sample powder into a 50mL centrifuge tube, add 25mL of 0.1% glacial acetic acid solution, vortex and mix, and store at 60°C Ultrasonic extraction for 20min, put it at room temperature, centrifuge at 10000r / min for 15min, take the supernatant and pass it over a filter paper covered with 0.5g activated carbon, collect the filtrate and pass through C 18 The solid phase extraction column was purified and passed through a 0.22 μm filter membrane.

[0039] Determination of samples: add 8mL sample solution, 550μL methylene blue solution (50μg / L), 1mL acetic acid-sodium acetate buffer solution with pH=4.5 to a 15mL centrifuge tube in turn, dilute to 10mL, shake well, let stand for 2min, add 70 μL of methanol, 350 μL of dichloromethane, vortex e...

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Abstract

The invention discloses a colorimetric determination method for perchlorate in food through dispersive liquid-liquid microextraction-smart phone. The method comprises the following steps: taking methylene blue as a color developing agent, and reacting with ClO4<-> in an acidic system with the pH of 4.5 to generate a hydrophobic green ionic associate; then using methyl alcohol as a dispersing agent, taking methylene dichloride as an extracting agent, and using a dispersive liquid-liquid microextraction technology to extract and enrich the ionic associate into organic phase methylene dichloride;then using Color Grab software of an Android phone to shoot and obtain the RGB color strength value of organic phase, and the quantitative relationship between the RGB color strength of organic phaseand the concentration of ClO4<-> is determined according to the working curve of ClO4<->, so that quantitative determination of ClO4<-> is realized. The method is high in accuracy, high in sensitivity and small in using amount of an organic reagent, the cost of involved instrument equipment is low, the method is easy for field deployment and not high in the requirement on the technological levelof operating personnel, and the method is expected to be popularized and used as rapid detection equipment of perchlorate in food.

Description

technical field [0001] The invention relates to a detection method of perchlorate ions, in particular to a dispersion liquid-liquid microextraction-smart phone colorimetric method for determining perchlorate in food, which belongs to the technical field of food detection. Background technique [0002] As an environmental pollutant, perchlorate has high stability (refractory to degradation), high solubility, high diffusivity and continuous mobility. Exposure to the environment will lead to long-term pollution of water sources and soil. After enrichment Or transformation, enter the human body through breathing, food chain and other ways. Drinking water, vegetables and fruits, meat, tea, milk and its products, and beverages are all foods that Chinese residents consume daily, and perchlorate is detected in different levels of them. Drinking water, tea, spinach The content of perchlorate in foods such as cantaloupe and cantaloupe can reach the ppm level, which poses a greater ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 李婷婷彭波刘兴斌马正华曾文锦黎凯
Owner 酒泉市食品检验检测中心
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