Salt-tolerant denitrifying bacteria, strain preparation method thereof and application of bacteria

A technology of nitrifying bacteria agent and denitrifying bacteria, which is applied in the field of environmental microorganisms, can solve the problems of incomplete treatment of high-concentration nitrate waste liquid, secondary pollution and salt tolerance effect, and achieve accelerated domestication and start-up efficiency, efficient and stable treatment methods , stable effect

Active Publication Date: 2019-08-16
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to solve the problem of incomplete treatment of high-concentration nitrate waste liquid in the prior art, the existence of secondary pollution, and the poor salt-tolerant effect of conventional denitrification processes, the present invention provides a high-efficiency, high-salt-tolerant and high-concentration nitrate degradation denitrifying bacteria and their uses

Method used

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  • Salt-tolerant denitrifying bacteria, strain preparation method thereof and application of bacteria
  • Salt-tolerant denitrifying bacteria, strain preparation method thereof and application of bacteria

Examples

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Embodiment 1

[0062] Embodiment 1: the isolation identification of bacterial strain of the present invention:

[0063] (1) Enrichment, separation medium and expansion medium:

[0064] Enrichment medium: potassium nitrate 2g, magnesium sulfate heptahydrate 0.2g, dipotassium hydrogen phosphate 1.76g, potassium sodium tartrate 20g, ferrous sulfate heptahydrate 0.005g, calcium chloride 0.02g, ammonium chloride 0.63g, NaCl 5 %, distilled water 1000mL, pH value 7.5.

[0065] Separation medium: Potassium nitrate 2g, magnesium sulfate heptahydrate 0.2g, dipotassium hydrogen phosphate 0.5g, potassium sodium tartrate 20g, NaCl 5%, distilled water 1000mL, pH 7.0-7.2, solid medium plus agar 1.5% and 1mL bromine Musk thymol blue solution.

[0066] Fermentation medium: peptone 8g, yeast extract 4g, sodium chloride 10g, distilled water 1000mL pH value 7.5.

[0067] (2) Isolation and purification of Halomonas bacterial strain:

[0068] Weigh 5g of the acclimatized denitrification sludge into a 250mL an...

Embodiment 2

[0077] Embodiment 2: the preparation of halomonas bacterial agent

[0078] After the Halomonas was activated and cultivated, the Halomonas seed solution was inserted into the fermentation medium according to the inoculum size of 5%, the fermentation temperature was controlled at 35°C, the dissolved oxygen was 3 -10 4 cfu / L of the bacterial suspension, and fill it to finally obtain the halomonas microbial agent.

[0079] The composition of the fermentation medium is: peptone 10g / L, yeast extract 4g / L, sodium chloride 35g / L, the balance is distilled water, pH value 7.5, sterilized at 121°C for 20min, cooled to room temperature after sterilization.

Embodiment 3

[0080] Embodiment 3: the preparation of halomonas bacterial agent

[0081] The salt-tolerant denitrifying bacteria were inoculated into the seed culture medium, the culture temperature was controlled at 35° C., and the culture was shaken at 35 rpm for 48 hours to obtain a seed fermentation liquid.

[0082] Put the halomonas seed fermentation liquid into the fermentation medium according to 10% inoculum amount, control the fermentation temperature at 35°C, dissolved oxygen <0.2mg / L, tank pressure 0.03-0.10Mpa, and shake at 40rpm for 72h.

[0083] After the Halomonas was cultured by fermentation, centrifuge at 7500 rpm for 10 min at 4°C, discard the supernatant to obtain a bacterial cell precipitate, dilute the bacterial cell precipitate with a fermentation medium, and obtain a bacterial activity of 10 3 cfu / L of the bacterial suspension, and fill it to finally obtain the halomonas microbial agent.

[0084] The seed medium consists of: KNO 3 2g / L, MgSO 4 ·7H 2 O 0.2g / L, K ...

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Abstract

The invention discloses salt-tolerant denitrifying bacteria, a strain preparation method thereof and an application of the bacteria, and belongs to the field of environmental microorganisms. The bacteria are named as Halomonas stevensii and belong to anaerobic denitrifying bacteria strains, and the collection number of the denitrifying bacteria is CGMCC NO. 15311. The Halomonas stevensii has highsalt-tolerant and denitrification characteristics, denitrification rate reaches 98% or more, high-concentration nitrate nitrogen in water can be reduced into harmless nitrite-free nitrogen under the condition of sodium chloride salinity lower than 10%, the denitrifying bacterium is applied into a denitrifying denitrification reactor, denitrification is effective and stable, secondary pollution isavoided, a start period can be shortened, and the denitrifying bacteria can be widely applied to denitrification treatment of industrial wastewater.

Description

[0001] Technical field: [0002] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant denitrifying bacterium and a preparation method and application thereof. [0003] Background technique: [0004] With the development of industrial and agricultural production and the improvement of people's living standards, the discharge of nitrogen-containing pollutants in my country has increased rapidly, and nitrogen-containing pollutants are one of the main sources of eutrophication in water bodies. According to rough statistics, about 30 million people in my country drink high-nitrate water, and nitrate pollution has become one of the main environmental factors of cancer in our country. After nitrate is ingested in humans and animals, part of it will be reduced to nitrite. Nitrite can oxidize the hemoglobin in the blood to methemoglobin, which does not have the ability to bind oxygen. When the content of methemoglobin in the b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC12N1/20C02F3/34C02F2101/163C12R2001/01C12N1/205
Inventor 刘锐平苗时雨柏耀辉胡承志王青山晋超曲久辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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