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Method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles

A nanoparticle, polypeptide modification technology, applied in chemical instruments and methods, instruments, analytical materials, etc., can solve problems such as limited applications

Active Publication Date: 2019-08-16
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, so far, the surface of AIE polymer nanoparticles prepared by miniemulsion polymerization has not been modified with specific peptides, which limits its application in the field of selective cell imaging to a certain extent.

Method used

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  • Method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles
  • Method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles
  • Method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Weigh 0.2g of O-50 emulsifier and 0.1g of 2-aminoethyl methacrylate hydrochloride, and dissolve them in 12.5g of deionized water to obtain an aqueous phase solution.

[0078] Weigh 0.01g of AIE-6 molecules and 0.02g of azobisisobutyronitrile and dissolve in a mixed solution of 0.06g of n-hexadecane and 0.9g of methyl methacrylate to obtain an oil phase solution; add the water phase solution to the oil phase The solution was pre-emulsified by stirring to obtain a coarse emulsion; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonication with a power of 400W for 9 minutes; nitrogen and oxygen were removed, and the temperature Raise to 65°C, react for 6h under nitrogen protection, and prepare amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change frequency was changed every 2h o...

Embodiment 2

[0095] Weigh 0.5 g of Tween 20 and 0.15 g of 2-aminoethyl methacrylate hydrochloride, dissolve them in 15 g of deionized water, and obtain an aqueous phase solution.

[0096] Weigh 0.0075g of AIE-12 molecules and 0.015g of azobisisoheptanonitrile and dissolve in a mixed solution of 0.08g of n-hexadecane and 1.35g of styrene to obtain an oil phase solution; add the water phase solution to the oil phase solution In the process, the coarse emulsion was prepared after stirring and pre-emulsification; the container containing the coarse emulsion was placed in an ice-water bath, and a stable monomer fine emulsion was obtained by ultrasonic wave with a power of 200W for 20 minutes; nitrogen and oxygen were removed, and the temperature rose to 60° C., under the protection of nitrogen, and react for 12 hours to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for three days, and the water change ...

Embodiment 3

[0102] Weigh 0.3g of MOA-9 and 0.05g of 2-aminoethyl methacrylate hydrochloride, dissolve them in 15g of deionized water, and obtain an aqueous phase solution.

[0103] Weigh 0.05g of the AIE-15 molecule and 0.05g of azobisisobutyronitrile and dissolve in a mixed solution of 0.08g of n-hexadecane, 0.45g of styrene and 0.5g of methyl methacrylate to obtain an oil phase solution; The solution is added to the oil phase solution, and the coarse emulsion is obtained after stirring and pre-emulsification; the container containing the coarse emulsion is placed in an ice-water bath, and the ultrasonic wave with a power of 600W is used for 5 minutes to obtain a stable monomer fine emulsion; Nitrogen was used to remove oxygen, the temperature was raised to 60° C., and under the protection of nitrogen, the reaction was carried out for 24 hours to prepare an amino-modified AIE polymer nanoparticle emulsion. With a molecular weight cut-off of 5000g·mol –1 The dialysis bag was dialyzed for...

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Abstract

A method for preparing amino acid-and-polypeptide-modified AIE polymer nanoparticles comprises the following steps: (1) dissolving an emulsifier and an amino functional monomer in water to obtain an aqueous solution; (2) dissolving an AIE molecule, an Ostwald ripening effect inhibitor and an oil-soluble initiator in a hydrophobic monomer to obtain an oil phase solution; (3) adding the aqueous solution to the oil phase solution, stirring and pre-emulsifying the obtained mixture to obtain a crude emulsion, carrying out ultrasonic treatment to obtain a fine monomer emulsion, introducing nitrogento remove oxygen, and carrying out a reaction to produce an amino-modified AIE polymer nanoparticle emulsion; (4) dissolving omega-maleimido alkyl acid and a carbodiimide condensing agent in an acidicpH buffer solution, and performing activation to obtain an activated intermediate solution; (5) adding the activated intermediate solution to the emulsion prepared in step (3), and carrying out a reaction to obtain a maleimide-modified AIE polymer nanoparticle emulsion; and (6) adding an aqueous solution of a polypeptide containing a cysteine sequence unit at the end to the emulsion prepared in step (5), and carrying out a reaction to produce the amino acid-and-polypeptide-modified AIE polymer nanoparticles.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing surface-modified aggregation-induced emission (AIE) polymer nanoparticle. [0003] (2) Background technology [0004] Fluorescent nanoparticles are an important class of functional nanomaterials, which have the characteristics of good water dispersibility, high photostability, low cytotoxicity, and surface modification. They are important in many high-end fields such as cell imaging, disease diagnosis, and biosensing. application value. However, nanoparticles with traditional aggregation-induced quenching (ACQ) fluorescent molecules as functional components often have defects such as low fluorescence quantum yield and narrow fluorescence intensity control range, which limits their application to some extent. In 2001, Academician Tang Benzhong's research group first reported a new type of fluorescent molecule with AIE effect, which emits strong fluorescence due to the restricted rotation within ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/14C08F220/34C08F222/40C08F2/44C09K11/06G01N21/64
CPCC08F2/44C08F220/14C09K11/06C09K2211/1425C09K2211/1458C09K2211/1466C09K2211/1483C09K2211/1491G01N21/6486C08F220/34C08F222/40
Inventor 曹志海胡亚新楼俊杰梁小琴肖立圣齐琪秘一芳
Owner ZHEJIANG SCI-TECH UNIV
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