Method for evaluating activity degree of formaldehyde dehydrogenase in plant leaves

A formaldehyde dehydrogenase, plant leaf technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of less research on formaldehyde dehydrogenase, and achieve the effects of low cost and complex operation.

Pending Publication Date: 2019-08-13
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Plants purify formaldehyde in the air as a safe, effective, and convenient green technology has received widespread attention, but most of the current research mainly focuses on superoxide dismutase, peroxidase and peroxidase in the mechanism of plant purification of formaldehyde. Research on catalase, less research on formaldehyde dehydrogenase in the mechanism of plant purification of formaldehyde

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Based on the evaluation of the activity of formaldehyde dehydrogenase in ivy leaves

[0020] (1) Prepare 15 10 mL stoppered colorimetric tubes, and add 0.5 mL of fresh plant extract to each of the 15 stoppered colorimetric tubes. Every three stoppered colorimetric tubes are set as a group in parallel, add 0μL, 100μL, 200μL, 300μL, 400μL formaldehyde (250 mg / L), 0.5mL acetylacetone, shake well, place in a 60℃ water bath and heat After 15 minutes, cool, filter, and dilute, and measure the absorbance of the filtrate at a wavelength of 414 nm with water as a reference;

[0021] (2) Prepare 15 10 mL stoppered colorimetric tubes, and add 0.5 mL plant inactivation extract to each of the 15 stoppered colorimetric tubes. Every three stoppered colorimetric tubes are set as a group in parallel, add 0μL, 100μL, 200μL, 300μL, 400μL formaldehyde (250 mg / L), 0.5mL acetylacetone, shake well, place in a 60℃ water bath and heat After 15 minutes, cool, filter, and dilute, and ...

Embodiment 2

[0023] Example 2 Based on the evaluation of the activity of formaldehyde dehydrogenase in Pangpengxiang leaves

[0024] Similar to Example 1, the difference is that the plants in steps (1) and (2) are replaced with Pangpengxiang with the same size and growth, and the additional formaldehyde purification amount of the fresh extract of Pengpengxiang leaves and the inactivated extract are respectively measured . The results showed that the purification amount of Pangpangxiang leaves was 35 mmol·kg -1 FW, where formaldehyde dehydrogenase can purify 7mmol kg of added formaldehyde -1 FW, the contribution rate of formaldehyde dehydrogenase in Pengpengxiang leaves to the added formaldehyde is 20%.

Embodiment 3

[0025] Example 3 Based on the evaluation of the activity of formaldehyde dehydrogenase in strawberry Chlorophytum leaves

[0026] Similar to Example 1, the difference is that the plants in steps (1) and (2) are replaced with strawberry Chlorophytum of the same size and growth, and the fresh extract of strawberry Chlorophytum leaves and the inactivated extract are respectively measured for additional formaldehyde purification. The results showed that formaldehyde dehydrogenase contributed 57 % to the added formaldehyde in the leaves of Chlorophytum chinensis.

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PUM

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Abstract

The invention discloses a method for evaluating the activity degree of formaldehyde dehydrogenase in plant leaves, and particularly relates to a method of removing formaldehyde in air by plants. The method adopts a high-temperature treatment mode, the activity of the formaldehyde dehydrogenase in a leaf extract solution is eliminated, and the activity degree of the formaldehyde dehydrogenase in the plant leaves is evaluated according to difference of the capability of removing the added formaldehyde between the fresh extract solution and an inactivated extract solution. When the concentrationof the added formaldehyde is 3.12 mmol / L, the contribution rate of the formaldehyde dehydrogenase in the plant leaves to the added formaldehyde is 1-73%. It is shown through experimental results thatthe contribution rate of hedera helix, plectranthus hadiensis var. Tomentosus and chlorophytum comosum to the added formaldehyde is 20-57%, and the method has great significance for the development ofrapid screening methods for efficiently purifying plants.

Description

technical field [0001] The invention relates to a method capable of evaluating the activity of formaldehyde dehydrogenase, in particular to the evaluation of the activity of formaldehyde dehydrogenase in plant leaves. Background technique [0002] Formaldehyde pollution caused by interior decoration has been widely concerned, and it is a serious problem that plagues the health of residents. Formaldehyde has a wide range of sources, great harm, and long duration. It has become one of the ubiquitous and serious indoor pollutants in my country. Its carcinogenicity has been confirmed by international authoritative organizations. At present, the ways to control indoor formaldehyde pollution mainly include physical methods, chemical methods, and biological methods. Due to the unsatisfactory effects and limitations of physical and chemical methods, the use of green plants to absorb formaldehyde from interior decoration has become a hot research topic today. [0003] Plants purify...

Claims

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Application Information

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IPC IPC(8): C12Q1/32
CPCC12Q1/32
Inventor 苏玉红杨玉霞赵苏亚
Owner XINJIANG UNIVERSITY
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