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A kind of bacterial rnase E truncated body and application thereof

A technology of bacteria and short body, applied in RNaseE truncation body and its application field, to achieve the effect of inhibiting growth and proliferation

Active Publication Date: 2022-06-07
GUIZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biological therapy such as tumor immunotherapy is also used in the clinical treatment of tumors, the effect still needs to be further improved

Method used

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  • A kind of bacterial rnase E truncated body and application thereof
  • A kind of bacterial rnase E truncated body and application thereof
  • A kind of bacterial rnase E truncated body and application thereof

Examples

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Embodiment 1

[0028] Example 1: A bacterial RNase E truncated body, the protein sequence of the bacterial RNase E truncated body is shown in SEQ ID NO.1.

[0029] A gene encoding a bacterial RNase E truncated body, the coding gene sequence of the bacterial RNase E truncated body is shown in SEQID NO.2.

[0030] A bacterial RNase E truncation encoding gene, a vector containing the bacterial RNase E truncation encoding gene: the linearized backbone vector pLVX-AcGFP-N1 and the target gene are double-enzyme digested with Xho I and EcoR I, and then connected, After transformation, screening, expansion and identification, the lentiviral gene expression vector pLVX-REt-AcGFP was obtained.

[0031] An application of a bacterial RNase E truncated body, and the application of the bacterial RNase E truncated body in tumor inhibition.

[0032] The preparation process steps of a bacterial RNase E truncated body are as follows:

[0033] 1. RNase E gene cloning and transformation

[0034] First, the E...

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PUM

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Abstract

The invention discloses a bacterial RNase E truncated body and its application. The protein sequence of the bacterial RNase E truncated body is shown in SEQ ID NO.1. The present invention utilizes the N-terminal of RNase E (amino acid residues 1-529) The functional domain of RNA can be degraded, and the nucleotide coding sequence of the truncated body (REt) is synthesized in vitro, and the SV40 nuclear localization sequence is added to its 5' end to make it expressed in the nucleus of cancer cells, and it is directly degraded in the nucleus, including mRNA in the nucleus. A variety of RNAs in the protein interfere with the biosynthesis of oncoproteins, thereby inhibiting the growth, proliferation, migration and invasion of cancer cells, and are expected to achieve the effect of inhibiting tumors.

Description

technical field [0001] The invention relates to a bacterial RNase E truncated body and application thereof, belonging to the technical field of RNase E truncated body and its application. Background technique [0002] The "2018 Global Cancer Statistics" report pointed out that in 2018, there were about 18.1 million new cancer cases and 9.6 million cancer deaths worldwide. Among the 18.1 million new cases, China's cancer incidence and mortality ranked first in the world. According to statistical analysis, in 2018, there were about 3.804 million new cases and 2.296 million deaths in my country, which means that for every 100 new cancer patients in the world, China accounted for 21. In my country, the top five cancers with the highest incidence are lung cancer, breast cancer, gastric cancer, colorectal cancer, and liver cancer. At present, although cancer treatment methods and methods are diversified, including surgical resection, chemotherapy, radiotherapy and biological immu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/22C12N15/55C12N15/867A61K48/00A61K38/46A61P35/00
CPCC12N9/22C12N15/86A61K48/005A61P35/00C12N2740/15043C12N2800/107A61K38/00
Inventor 孙达权石静余畅陈相屹石松徐莹莹胡桐张钦真
Owner GUIZHOU MEDICAL UNIV
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