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Absorbable starch microsphere hemostatic powder with antibacterial activity and application thereof

A technology of starch microspheres and antibacterial activity, applied in the field of medicine, can solve the problems of infection, excessive bleeding, high incidence, cross infection, etc., and achieve the effects of excellent hemostatic performance, good histocompatibility, and good antibacterial activity.

Inactive Publication Date: 2019-08-13
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Trauma and bleeding problems have a long history. Both the East and the West have their own traditional treatment methods, but the main problems such as infection and excessive bleeding have not been effectively solved for a long time
In addition, in most cases, trauma and infection always occur at the same time. Due to the impact of trauma environment and other factors, the incidence of post-injury infection is extremely high. At the same time, new secondary infection and cross-infection may be caused in the follow-up treatment
At present, the commonly used method to control bacterial infection is the application of antibiotics, but the antibacterial effect of antibiotics on local wounds is often limited, and there are also problems such as drug resistance, toxic side effects, and antibiotic abuse.

Method used

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  • Absorbable starch microsphere hemostatic powder with antibacterial activity and application thereof
  • Absorbable starch microsphere hemostatic powder with antibacterial activity and application thereof
  • Absorbable starch microsphere hemostatic powder with antibacterial activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 absorbable starch microspheres

[0041] sample 1

[0042] Dissolve 5 g of soluble starch in 50 mL of NaOH solution (NaOH:H 2 (0=1:100, w / w) was made into a starch solution; 0.5 g of sodium trimetaphosphate was added into the above starch solution and stirred until completely dissolved. Add 7.5g of emulsifier Span 80 and 150mL of liquid paraffin into a 250mL three-necked flask, install a reflux condenser on the three-necked flask, put it into a constant temperature water bath, and heat it at 50°C while performing magnetic stirring; wait until the emulsifier is completely dissolved And after the temperature of the oil phase is stabilized at 40°C, take 15 mL of the prepared sodium starch trimetaphosphate solution and add it dropwise to the three-necked flask at a speed of 600 rpm. Keep stirring at this speed for 5 hours; stop stirring, transfer the emulsion in the three-necked flask into a centrifuge tube, centrifuge and discard the upper ...

Embodiment 2

[0052] The preparation of embodiment 2 antibacterial absorbable starch microsphere hemostatic powder

[0053] sample 1

[0054] Dissolve 2.5 mg of lysozyme in double-distilled water, add 100 mg of the starch microspheres prepared in Example 1, stir for 12 hours, add the above liquid into a centrifuge tube, centrifuge and discard the supernatant, dehydrate the lower layer with ethanol, and vacuum at room temperature After drying, the white powder obtained is the starch microspheres loaded with lysozyme. After determination, every 100g of starch microspheres contains 0.5g of lysozyme.

[0055] sample 2

[0056] Dissolve 12.5 mg of lysozyme in double-distilled water, add 100 mg of the starch microspheres prepared in Example 1, stir for 12 hours, add the above liquid into a centrifuge tube, centrifuge and discard the supernatant, dehydrate the lower layer with ethanol, and vacuum at room temperature After drying, the white powder obtained is the starch microspheres loaded with ...

Embodiment 3

[0061] Determination of water absorption swelling degree of embodiment 3 starch microspheres and antibacterial starch microspheres hemostatic powder

[0062] Accurately weigh 5 parts each of the same amount of starch microspheres prepared in Example 1 and 4 kinds of lysozyme-loaded starch microspheres prepared in Example 2, place them in centrifuge tubes respectively, add 3ml of distilled water with a pipette, and centrifuge 3, 5, 10, 20 and 30 minutes, remove the upper layer of distilled water, absorb the water on the surface of the sample with absorbent paper, weigh the wet weight (calculated as W wet), then put it in a 40°C oven to dry to constant weight, and weigh the dry weight again ( Count as W dry). According to the formula SW=(W wet-W dry) / W dry * 100%, calculate the swelling degree SW of porous starch foam, the result sees Figure 5 . The water absorption rate of the antibacterial starch microsphere hemostatic powder loaded with different amounts of lysozyme is 500...

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Abstract

The invention discloses starch microsphere hemostatic powder with antibacterial activity and application thereof. The preparation method of the hemostatic powder comprises the following steps of adopting soluble starch as a raw material and sodium trimetaphosphate as a crosslinking agent for preparing starch microspheres with a three-dimensional network structure by means of a reverse emulsification crosslinking approach; adding the prepared starch microspheres into a lysozyme solution to obtain the lysozyme-loaded antibacterial starch microsphere hemostatic powder. The preparation method is simple, and the prepared lysozyme-loaded antibacterial starch microsphere hemostatic powder has excellent hemostatic performance, high bacteriostatic activity and high biocompatibility and can be degraded and absorbed by the human body.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a novel antibacterial hemostatic material and its preparation method and application, in particular to a starch microsphere hemostatic powder with antibacterial activity and its application. Background technique [0002] In the process of surgical operation and accidental trauma treatment, bleeding and oozing are problems that must be solved, especially in the process of performing operations on multi-vascular parenchymal organs with rich blood supply (such as liver, spleen, etc.). Bleeding and oozing are also unavoidable. If it is not handled carefully, it will seriously endanger the life of the patient. Therefore, rapid and effective hemostasis treatment on the bleeding area of ​​the patient to reduce blood loss is a necessary means to reduce the pain of the patient, shorten the operation time, and reduce the mortality rate of the patient. Trauma and bleeding problems have a lo...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/08A61L24/00A61K38/47A61K9/14A61K47/36A61P31/02A61P31/04A61P7/04
CPCA61K9/146A61K38/47A61L24/001A61L24/0015A61L24/0042A61L24/08A61L24/108A61L2300/254A61L2300/404A61L2300/418A61L2400/04C12Y302/01017C08L3/02C08L89/00
Inventor 王思玲高亦鲲张景海岳扬
Owner SHENYANG PHARMA UNIVERSITY
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