Preparation method of carboxyl and polypeptide modified AIE polymer nanoparticles

Abstract

The invention relates to a preparation method of carboxyl and polypeptide modified AIE polymer nanoparticles, the method comprises the following steps: 1) preparing an emulsifier aqueous solution; 2)dissolving AIE molecules and an Austrian ripening effect inhibitor in a mixed solution of carboxyl functional monomers and hydrophobic monomers to obtain an oil phase solution; 3) adding the emulsifier aqueous solution into the oil phase solution, stirring and pre-emulsifying to obtain crude emulsion, and performing ultrasonic treatment to obtain monomer miniemulsion; introducing nitrogen to remove oxygen, adding a water-soluble initiator to react; 4) dissolving a carbodiimide condensing agent in an acidic pH buffer solution, and adding the carbodiimide condensing agent into the emulsion prepared by the step 3) for activation; 5) dissolving omega-aminomaleimide in an alkaline pH buffer solution to obtain an omega-aminomaleimide solution; 6) adding the omega-aminomaleimide solution to the emulsion prepared in the step 4) for reaction; and (7) adding a polypeptide aqueous solution containing cysteine sequence units at the tail end to the emulsion prepared in the step (6) for reaction toprepare the carboxyl and polypeptide modified AIE polymer nanoparticles.

Description

Technical field [0001] The invention relates to a method for preparing surface-modified aggregation-induced luminescence (AIE) polymer nanoparticles. Background technique [0002] Fluorescent nanoparticles are an important class of functional nanomaterials. They have the characteristics of good water dispersibility, high photostability, low cytotoxicity and surface modification. They are important in many high-end fields such as cell imaging, disease diagnosis and biosensing. Application value. However, nanoparticles with traditional aggregation-induced quenching (ACQ) fluorescent molecules as functional components often have defects such as low fluorescence quantum yield and narrow fluorescence intensity control range, which limits their application to a certain extent. In 2001, the research group of Academician Tang Benzhong first reported a new type of fluorescent molecule with AIE effect. In the aggregated state, due to the limited rotation of the molecule, it will emit stro...

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Problems solved by technology

So far, the surface of AIE polymer nanoparticles prepared by miniemulsion polymerization has not been modified with specific peptides, which limits its application value in the field of selective cell imaging

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