Histone acetyltransferase timing-current sensor based on gold-palladium nanoflower/graphene composite material and application thereof

A technology of acetyltransferase and current sensor, applied in the field of functional biomaterials and biosensing, to achieve high-sensitivity detection, fast detection speed, and good selectivity

Active Publication Date: 2019-08-02
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no related reports on the preparation and application of histone acetyltransferase timing-current sensors based on gold-palladium nanoflowers / graphene composites at home and abroad

Method used

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  • Histone acetyltransferase timing-current sensor based on gold-palladium nanoflower/graphene composite material and application thereof
  • Histone acetyltransferase timing-current sensor based on gold-palladium nanoflower/graphene composite material and application thereof
  • Histone acetyltransferase timing-current sensor based on gold-palladium nanoflower/graphene composite material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of sensor

[0032](1) Acetyl antibody-gold palladium nanoflower / graphene composite material (Ab Ac - Preparation of AuPd@GO)

[0033] Disperse 0.5g graphene (GO) in 20mL deionized water and sonicate for 0.5h, then add 2.5mL 1g / L chloroauric acid (HAuCl 4 ) solution and 2.5mL 1g / L of chloropalladium acid (H 2 PdCl 4 ) solution, stirred evenly by magnetic force for 0.5h, placed under xenon arc lamp irradiation for 0.5h, then transferred to a drying oven, and reacted at 60°C for 24h. Finally, the sample was placed in a hydrogen tube furnace at 300 °C for 2 h. Disperse the prepared AuPd@GO in deionized water to obtain a 1 mg / mL AuPd@GO dispersion for later use.

[0034] Take 100 μL 1g / L acetyl antibody (Ab Ac ) placed in a He-Ne laser therapeutic apparatus for 30s irradiation, then added to 1mL of the above-mentioned AuPd@GO dispersion, and reacted at 37°C for 4h.

[0035] (2) Preparation of histone acetyltransferase timing-amperometric senso...

Embodiment 2

[0043] Example 2 Electrochemical response with or without p300

[0044] The histone acetyltransferase chrono-current sensor based on the gold-palladium nanoflower / graphene composite material and its application, using Example 1 to prepare our biosensor to explore the detection of histone acetyltransferase. See figure 2 , In the absence of p300, the sensor has basically no electrochemical response in PBS (0.1M, pH 7.0), while in the presence of p300, there is an obvious electrochemical response, which proves that the sensor can be used for p300 activity detection.

Embodiment 3

[0045] Example 3 Detection of p300 activity

[0046] Histone acetyltransferase timing-current sensor based on gold-palladium nanoflower / graphene composite material and its application, the preparation steps of the sensor are the same as in Example 1, during the acetylation reaction, the concentration of p300 and the concentration of p300 are changed successively For: 0, 0.001, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20, 50, 100, 150, 200, 300, 500, 700, 1000 nM, subsequently used in the preparation sensor. Record the electrochemical response of the sensor in PBS (0.1M, pH 7.0). According to the experimental results, a series of electrochemical response curves corresponding to different concentrations of p300 are obtained, and the quantitative relationship between the electrochemical response current and the concentration of p300 is established. The quantitative relationship between the two determines the concentration of p300 in the sample to be tested. Experime...

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Abstract

The invention discloses a histone acetyltransferase timing-current sensor based on gold-palladium nanoflower / graphene composite material and an application thereof. Firstly, substrate polypeptide is fixedly disposed on a surface of a gold electrode by using Au-S effect, and acetyl group on acetyl-CoA is transferred to the specific lysine residue of the substrate polypeptide by acetylation reaction. The generated acetylated polypeptide is used to specifically adsorb the acetyl antibody-gold palladium nanoflower / graphene composite material with stronger catalytic capability, and obvious electrochemical signals are generated in electrolyte solution containing hydrogen peroxide. During the acetylation reaction, p 300 concentration and small molecule inhibitor concentration thereof are changed,and the influence on the electrochemical signals of a series of prepared sensors is explored through specific binding effect of acetyl and acetyl antibodies. The histone acetyltransferase timing-current sensor has advantages of good specificity, high sensitivity, fast detection speed, reliable and accurate result, and low costs.

Description

technical field [0001] The invention relates to a timing-current sensor and its application, in particular to an electrochemical detection method for histone acetyltransferase based on a gold-palladium nanoflower / graphene composite material, which belongs to the technical field of functional biological materials and biosensing. Background technique [0002] Post-translational modifications of histones, such as acetylation, phosphorylation, methylation, ADP-ribosylation, ubiquitination, etc., play an important role in the physiological and pathological processes of eukaryotic organisms. Among them, the acetylation of histone is mainly catalyzed by histone acetyltransferase (HAT), which uses acetyl-CoA as the reaction substrate to carry out enzymatic reaction, hydrolyzes the acetyl group on acetyl-CoA, and transfers it to histone On tail-specific lysine residues, this is a reversible process. p300 is a typical histone acetyltransferase, which has a wide range of biological fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327G01N33/543G01N33/573C01B32/182B82Y30/00B82Y15/00B01J23/52
CPCB01J23/52B01J35/0033B82Y15/00B82Y30/00C01B32/182G01N27/26G01N27/3278G01N33/5438G01N33/54386G01N33/573
Inventor 胡宇芳张青青胡丹丹郑宇迪冉平建刘鑫达郭智勇王邃晁多斌
Owner NINGBO UNIV
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