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Metal polyphenol microcapsule carrier with ROS (reactive oxygen species) responsiveness and metal ion slow-release function and preparation method

A metal ion and responsive technology, applied in drug delivery therapy, microcapsule carrier, and biomedical fields, achieves the effect of simple process, good scavenging ability, and mild preparation conditions

Pending Publication Date: 2019-08-02
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of metal polyphenol microcapsule carriers with ROS responsiveness and metal ion sustained release function.

Method used

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  • Metal polyphenol microcapsule carrier with ROS (reactive oxygen species) responsiveness and metal ion slow-release function and preparation method
  • Metal polyphenol microcapsule carrier with ROS (reactive oxygen species) responsiveness and metal ion slow-release function and preparation method
  • Metal polyphenol microcapsule carrier with ROS (reactive oxygen species) responsiveness and metal ion slow-release function and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing a metal polyphenol microcapsule carrier with ROS responsiveness and metal ion slow-release function, comprising the following steps:

[0035] (1) Dissolve 1000mg of calcium chloride dihydrate in 30ml of water to obtain solution 1; dissolve 1000mg of anhydrous sodium carbonate in 30ml of water to obtain solution 2: add all of solution 2 to the entire stirring solution 1, and continue stirring for 30s , stand still for 5 minutes, centrifuge at 8000rpm for 4min, wash the precipitate with water twice, 30mL each time, and centrifuge again; the stirring speed is 500rpm;

[0036] (2) Add water to the precipitate obtained in step (1) to 10mL; add 2mL of 50mg / mL water as a solvent and a liquid prepared with catechol, and mix well; add 2mL of 10mg / mL copper ion (CuCl 2 ) aqueous solution, mix well, add 10mL of 10mMpH=7.4 3-(N-morpholine)propanesulfonic acid aqueous solution, mix well, under the condition of 8000rpm, centrifuge for 8min; wash with water twic...

Embodiment 2

[0043] A method for preparing a metal polyphenol microcapsule carrier with ROS responsiveness and metal ion slow-release function, comprising the following steps:

[0044] (1) Dissolve 10 mg of calcium chloride dihydrate in 10 ml of water to obtain solution 1; dissolve 10 mg of anhydrous sodium carbonate in 10 ml of water to obtain solution 2: add all of solution 2 to the entire stirring solution 1, and continue stirring for 10 seconds , stand still for 1min, centrifuge at 2000rpm for 3min, wash the precipitate with water once, 1mL each time, and centrifuge again; the stirring speed is 400rpm;

[0045] (2) Add water to the precipitate obtained in step (1) to 4mL; add 0.5mL 1mg / mL of water as a solvent, liquid prepared with tannic acid, and mix; add 0.5mL 0.5mg / mL iron ion ( FeCl 3 ) aqueous solution, mix well, add 0.5mL 1mMpH=7.4 3-(N-morpholine)propanesulfonic acid aqueous solution, mix well, under the condition of 2000rpm, centrifuge for 5min; wash with water once, and cent...

Embodiment 3

[0052] A method for preparing a metal polyphenol microcapsule carrier with ROS responsiveness and metal ion slow-release function, comprising the following steps:

[0053](1) Dissolve 2000mg of calcium chloride dihydrate in 50ml of water to obtain solution 1; dissolve 2000mg of anhydrous sodium carbonate in 50ml of water to obtain solution 2: add all of solution 2 to the entire stirring solution 1, and continue stirring for 60s , stand still for 10 minutes, centrifuge at 13000rpm for 5min, wash the precipitate with water 3 times, 50mL each time, and centrifuge again; the stirring speed is 700rpm;

[0054] (2) add water to 20mL in the precipitation that step (1) obtains; Add 5mL of 100mg / mL water as solvent, the liquid prepared with pyrogallol, mix; Add 5mL 50mg / mL zinc ion (Zn( NO) 2 ) aqueous solution, mix well, add 50mL of 20mM 3-(N-morpholine)propanesulfonic acid aqueous solution with pH=7.4, mix well, centrifuge at 13000rpm for 10min; wash with water 3 times, and centrifu...

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Abstract

The invention discloses a metal polyphenol microcapsule carrier with ROS (reactive oxygen species) responsiveness and metal ion slow-release function and a preparation method. The preparation method comprises the following steps of (1) dissolving calcium chloride dehydrate into water, so as to obtain a solution I; dissolving calcium carbonate anhydrous into water, so as to obtain a solution II; adding the solution II into the stirred solution I, precipitating, washing by water, and centrifuging; (2) adding water into the precipitate, adding liquid prepared from polyphenol, and uniformly mixing; adding an aqueous solution of metal ions, uniformly mixing, adding an aqueous solution of 3-(N-morpholine)propanesulfonic acid, uniformly mixing, centrifuging, washing by water, and centrifuging, soas to obtain precipitate; (3) repeating the step (2); (4) adding an aqueous solution of EDTA (ethylene diamine tetraacetic acid), uniformly mixing, standing, and centrifuging, so as to obtain precipitate; (5) repeating the step (4); (6) washing the precipitate by water; mixing and suspending in water, and freeze-drying. Proofed by experiment, the metal polyphenol microcapsule carrier has good ability of clearing H2O2 (hydrogen peroxide); proofed by a mouse lower limb ischemia model, the metal polyphenol microcapsule carrier has the effect of accelerating generation of micro-blood vessels.

Description

technical field [0001] The invention relates to a metal polyphenol microcapsule with ROS responsiveness and metal ion sustained release function, a preparation method and an application in the preparation of drugs for treating ischemia of lower limbs, and belongs to the fields of biomedicine, microcapsule carrier and drug delivery treatment. Background technique [0002] The incidence of ischemic diseases of the lower extremities is high, and the annual amputation rate of severe patients is as high as 30%. At present, there are problems such as postoperative vascular restenosis or occlusion in the clinical operation and interventional treatment of such diseases, and the effect of gene and stem cell therapy is not ideal, and the cost is high; therefore, it is necessary to effectively improve the ischemic microenvironment and induce angiogenesis. key to treating these diseases. In recent years, research on biomaterial technology has covered a variety of disease areas. Metal ...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/02A61K47/42A61K47/36A61P9/14
CPCA61K9/501A61K9/5036A61K9/5057A61P9/14
Inventor 王晓莉杨菁李拥军段建伟陈作观
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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