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A kit for rapid detection of antibodies against natural echinococcosis in animals

A technology for detecting animals and natural infections, applied in biological tests, measuring devices, material inspection products, etc., can solve problems such as difficulties in diagnosing hydatid disease in livestock and inability to distinguish vaccine immune antibodies from antibody detection reagents, and achieves huge market value. The effect of far-reaching and accurate differential diagnosis

Inactive Publication Date: 2021-12-21
SHENZHEN ZHENRUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since my country's sheep echinococcosis genetically engineered vaccine was officially approved for marketing in 2012, and began to be widely used in large quantities, traditional antibody detection reagents cannot distinguish vaccine immune antibodies from antibodies produced by natural infection, which is difficult for the diagnosis of livestock echinococcosis. brought new difficulties and problems

Method used

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  • A kit for rapid detection of antibodies against natural echinococcosis in animals
  • A kit for rapid detection of antibodies against natural echinococcosis in animals
  • A kit for rapid detection of antibodies against natural echinococcosis in animals

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Embodiment 1

[0027] see Figure 1-3 , in an embodiment of the present invention, a kit for rapidly detecting antibodies against natural hydatid infection in animals, comprising a colloidal gold test paper card and a sample diluent, the colloidal gold test paper card includes a closed box body 2 and a box body 2 laid flat on the box The reaction test strip 1 inside the body 2, the box body 2 is respectively provided with an observation window 3 and a sampling hole 4 connected to the reaction test strip 1; the sample diluent is composed of 0.01M pH7.2 A mixed solution of PBS and 1% tween-80.

[0028] Further, the box body 2 includes an upper box body and a lower box body arranged in cooperation, and the observation window 3 and the sample injection hole 4 are arranged on the upper box body of the box body 2 .

[0029] Further, the sample diluent is stored in a 5 ml drop bottle.

[0030] Further, the reaction test strip 1 includes a bottom plate 11, a sample pad 12 arranged on it along the ...

Embodiment 2

[0037] see Figure 4 , in the embodiment of the present invention, the application method of the kit for rapid detection of animal echinococcosis natural infection antibody is as follows:

[0038] 1. Antigen purification

[0039] The purification of the antigen first uses SPG gel chromatography column to remove the host antibody components. Then, use molecular sieve chromatography to group them according to the peaks of molecular size, and use SDS-PAGE gel electrophoresis for each histone to remove the group where the Eg95 antigen resides.

[0040] 2. Monoclonal antibody preparation and screening

[0041]The core key technology lies in the preparation and screening of specific monoclonal antibodies. Use the purified hydatid vesicle crude antigen to immunize BALB / C mice, remove the spleen of the immunized mice, disperse the spleen cells, make them fuse with myeloma cells, and culture them monodispersely, and the hybridoma cells are covered with the bottom of the well At 1 / 1...

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Abstract

The invention relates to the field of immune colloidal gold chromatography analysis, in particular to a kit for rapidly detecting antibodies against natural hydatid infection in animals. The kit can specifically detect antibodies against natural hydatid infection and has no cross-reaction with vaccine immune antibodies. Including a colloidal gold test paper card and a sample diluent, the colloidal gold test paper card includes a closed box body and a reaction test strip laid on the inside of the box body, and the box body is respectively opened with connections to the reaction test strips. The observation window and sample injection hole of the paper strip; the sample diluent is a mixed solution comprising 0.01M PH7.2 PBS and 1% tween-80. The invention provides a simple, rapid and scientific method for diagnosing echinococcosis infection, which can quickly detect whether the blood sample contains the natural infection antibody of animal echinococcosis.

Description

technical field [0001] The invention relates to the field of immunocolloid gold chromatography analysis, in particular to a kit for rapidly detecting antibodies against natural echinococcosis infection in animals, which is used for detecting antibodies against natural echinococcosis infection in animal whole blood or serum samples. Background technique [0002] Because echinococcosis is a kind of zoonotic parasitic disease, and the high infection rate in domestic dog and domestic animal simultaneously, it is necessary to reduce the infection rate of domestic dog and domestic animal at first to prevent and treat human echinococcosis, and to diseased animal Diagnosis and screening are the first steps. [0003] The traditional diagnostic method of livestock echinococcosis mainly uses immunological detection kits to detect anti-hydatid antibodies in the blood of infected animals. However, since my country's sheep echinococcosis genetically engineered vaccine was officially appr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
CPCG01N33/5304
Inventor 饶乐
Owner SHENZHEN ZHENRUI BIOTECH CO LTD
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