Ageratum conyzoides extract, application in fish feed, fish feed and preparation method thereof
A technology of Agaricus and extract, applied in the field of fish feed, can solve the problem that the mechanism of action is not studied, and achieve the effects of improving food intake and feed efficiency, good extraction effect, and improving intestinal lipase and amylase activities.
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Embodiment 1
[0051] Obtain 1000 g of the whole plant of Ageratum marianum, dry it in a forced-air drying oven at 50° C. to constant weight, grind it through a 18-mesh sieve, and obtain Ageratum marianum powder.
[0052] Take 400g of ageratum powder, mix ageratum powder and petroleum ether according to the ratio of 1:7-9, rotate and stir at 800 rpm for 6 hours; filter the mixture to obtain a clear filtrate and residue, and repeatedly extract the residue The filtrates were combined three times to obtain residue I; the filtrate was distilled under reduced pressure to constant weight to remove petroleum ether, and the ageratum petroleum ether extract was obtained. Mix the residue Ⅰ after extraction of ageratum petroleum ether with ethyl acetate at a ratio of 1:7-9, rotate and stir at 800 rpm for 6 hours; filter the mixture to obtain a clear filtrate and residue, and repeat the extraction of the residue The filtrates were combined three times to obtain residue II; the filtrate was distilled und...
Embodiment 2
[0064] In order to explore the effect of ageratum extract as a fish feed additive on fish growth, the following experiments were conducted:
[0065] In the first step, the ageratum extract is extracted according to the extraction method of Example 1;
[0066] Step 2: According to the feed formula in Table 2 below, add the 4 kinds of ageratum extracts to the feed raw materials according to the concentration of 0 and 2g / kg feed and mix them to make pellet feed with a diameter of 2mm.
[0067] Table 2 feed formula
[0068]
[0069]
[0070] 1 It is homemade without any additives.
[0071] 2 Each kilogram of vitamin supplements contains: retinol (500,000IU / g) 0.80g, vitamin D 3 (500,000IU / g) 0.48g, DL-α-tocopherol (50%) 20.00g, vitamin K 3 (23%) 0.43g, Vitamin B 1 (90%) 0.11g, riboflavin (80%) 0.63g, pyridoxine (81%) 0.92g, cyanocobalamin (1%) 0.10g, D-calcium pantothenate (90%) 2.73g, niacin (99%) 2.82g, D-biotin (2%) 5.00g, inositol (99%) 52.33g and folic acid (96%)...
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