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HPLC standard fingerprint of pterocarpus santalinus, and constructing method and application thereof

A standard fingerprint, sandalwood and red sandalwood technology, applied in measuring devices, instruments, scientific instruments and other directions, can solve the problems of damaged samples, sandalwood and red sandalwood authenticity identification does not have an objective and unified standard, etc., and achieve accurate and reliable results.

Inactive Publication Date: 2019-07-19
汕头出入境检验检疫局检验检疫技术中心
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In order to solve the problem that the authenticity of sandalwood and red sandalwood in the prior art does not have an objective and unified standard, the result is greatly affected by subjective uncertain factors, and it will damage the sample

Method used

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  • HPLC standard fingerprint of pterocarpus santalinus, and constructing method and application thereof
  • HPLC standard fingerprint of pterocarpus santalinus, and constructing method and application thereof
  • HPLC standard fingerprint of pterocarpus santalinus, and constructing method and application thereof

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings.

[0022] 1. Establish the HPLC standard fingerprint of sandalwood and rosewood.

[0023] Agilent 1200 high performance liquid chromatography (USA): DAD detector, quaternary gradient pump; MilliporeAdvantage A10 ultrapure water system (USA); Mettler-Toledo AL204-IC electronic analytical balance (Switzerland); CBLphotoelectron TECHNOLOGY Model C9860A ultrasonic cleaner.

[0024] The acetonitrile used for the liquid phase is chromatographically pure; the water used for the liquid phase is ultrapure water; the rest of the reagents are analytically pure.

[0025] Chromatographic conditions: Chromatographic column: Agilent Eclipse XDB-C18, packing particle size 5μm, column length 250mm, column inner diameter 4.6mm;

[0026] Mobile phase: use water as mobile phase A, acetonitrile as mobile phase B, perform gradient elution according to Table 1; detection wavelength: 290nm; co...

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Abstract

The invention discloses an HPLC standard fingerprint of pterocarpus santalinus. The HPLC standard fingerprint has six characteristic peaks, wherein the peak area of the characteristic peak with the longest retention time is the largest; by taking the characteristic peak with the largest peak area as the reference pea, the relative retention times tg of the six characteristic peaks are respectively0.085, 0.099, 0.133, 0.160, 0.271 and 1.000, and RSD% is less than 2%. The invention also discloses a constructing method of the fingerprint. The HPLC standard fingerprint of pterocarpus santalinus established by the invention can effectively identify the authenticity of pterocarpus santalinus by similarity analysis and comparison of characteristic peaks.

Description

technical field [0001] The invention belongs to the technical field of wood identification, and relates to a sandalwood red sandalwood HPLC (high performance liquid chromatography) standard fingerprint and its construction method and application. application. Background technique [0002] Sandalwood red sandalwood (Pterocarpus santalinus) is a Papilionaceae, Pterocarpus trees, also known as Indian small leaf red sandalwood. Arbor, 8 to 11 meters high, mainly produced in India. Solid and thick, fine wood, high density, resistant to carving, dark purple or black purple in color. Sandalwood red sandalwood grows extremely slowly, and it takes hundreds or even thousands of years to grow. Because of its extremely low yield rate and scarcity of resources, it is expensive and ranks first among all trees. Driven by huge profits, counterfeit products of sandalwood and red sandalwood are rampant. How to accurately identify sandalwood red sandalwood has become the biggest problem in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686
Inventor 魏建华吴剑光魏霜刘玉莉刘碧琳
Owner 汕头出入境检验检疫局检验检疫技术中心
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