Microfluidic chip and analysis system

Abstract

The application relates to a microfluidic chip and an analysis system. The microfluidic chip comprises a chip matrix, a sample adding chamber, an air outlet, a sample enrichment and pyrolysis chamber,a first waste liquid chamber, a dilution chamber, a capillary valve, a second waste liquid chamber, a sample adding chamber circulation pipeline, a pyrolysis chamber circulation pipeline, an air circulation pipeline, a sample output pipeline, a reagent distribution pipeline and a plurality of PCR amplification chambers; the sample output pipeline is provided with a phase change valve and besidesthe chip matrix is provided with an encapsulation hole communicating with the phase change valve; and positioning areas are arranged in the chip matrix. The microfluidic chip is suitable for centrifugal microfluidic analysis and can realize a molecular diagnostic function of nucleic acid-free purification, the phase change valve can be conveniently placed in the sample output pipeline through theencapsulation hole to control entering of samples in the sample enrichment and pyrolysis chamber into the dilution chamber under certain conditions, a design of the positioning areas is convenient forconveniently and quickly positioning and fixing the microfluidic chip, the microfluidic chip is applied to a centrifugal microfluidic technology to realize a molecular diagnosis based on PCR amplification, and a whole reaction process is in a closed microfluidic chip structure.

Description

technical field [0001] This application relates to the field of centrifugal microfluidics, in particular to microfluidic chips and analysis systems. Background technique [0002] Microfluidics refers to the manipulation of liquids on the submillimeter scale, where the submillimeter scale is generally several microns to hundreds of microns. Microfluidic technology integrates the basic operating units involved in the fields of biology and chemistry, and even the functions of the entire laboratory, including sampling, dilution, reaction, separation, detection, etc., on a small chip, so it is also called lab-on-a-chip ( Lab-on-a-Chip). This kind of chip is generally composed of various liquid storage pools and interconnected microchannel networks, which can greatly shorten the sample processing time, and realize the maximum utilization efficiency of reagent consumables through precise control of liquid flow. [0003] Microfluidic systems are devices that manipulate liquids on ...

AI Technical Summary

Problems solved by technology

[0005] However, in the molecular diagnostic system based on PCR amplification, due to aerosol pollution during PCR amplification, and in order to avoid cross-contamination between samples, it is generally necessary to set up a partitioned laboratory

This laboratory needs to implement sample processing, nucleic acid extraction, and PCR amplification partition operations, and must have a good ventilation system. The cost of laboratory construction is high, and often only large medical institutions have the financial resources to build it.

On the other hand, laboratory operators have to hold certificates to work, which also greatly increases labor costs

At the same time, too much manual intervention will inevitably lead to human error

These issues have greatly raised the barriers to technical adoption of PCR-based molecular diagnostics

[0006] Moreover, in the current molecular diagnostic laboratory mode, multi-sample and multi-test project operations are completed in a centralized experimental site, and the process quality control requirements are high.

Moreover, the current molecular diagnostic laboratory mode is generally a multi-sample single-indicator detection mode, and the detection indicators are limited, so it is impossible to screen single-sample multi-indicator infectious pathogens

In addition, although the advantages of molecular diagnostic technology are obvious, molecular diagnostics are also expensive due to the cumbersome steps, time-consuming process, and the need for professionals to operate, and the construction cost of clinical molecular diagnostic laboratories is generally high.

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