Preparation method of nanocellulose bio-printing gel ink

Abstract

The invention discloses a preparation method of nanocellulose bioprinting gel ink. The preparation method comprises the following steps: pretreating pulp fibers by high-pressure sterilization and alkali / acid sequential extraction; then, performing TEMPO catalytic oxidation combined with high-pressure homogenization treatment to prepare ultrapure nanocellulose hydrogel; biocoupling sterilized xyloglucan and cell adhesion molecules to prepare bioactive xyloglucan; dissolving bioactive xyloglucan in sterile water; then, adding the nanocellulose hydrogel and a sterilized gel enhancer; and mixing uniformly to obtain ultrapure nanocellulose bio-printing gel ink. The prepared ink has excellent biocompatibility, no cytotoxicity and biodegradability; a highly hydrated three-dimensional network structure can simulate and restore a real extracellular matrix micro-environment and structure to the greatest extent and provide an ideal microenvironment for cell adhesion, growth, reproduction and differentiation; and the content of pyrogenic and sensitizing impurities in the prepared ink is far lower than pharmacopoeia regulation and medical clinical threshold, and is widely applied.

Description

technical field [0001] The invention relates to a preparation method of gel ink, in particular to a preparation method of ultrapure nano-cellulose bioprinting gel ink, and belongs to the technical field of manufacturing new biological and medical materials. Background technique [0002] Nanocellulose is easy to form hydrogel during the preparation process, and its highly hydrated three-dimensional network structure can well simulate the extracellular matrix in human organ tissues, thereby providing a good 3D microenvironment for cell adhesion, growth, and reproduction. Therefore, the current development and utilization of 3D printing cellulose-based materials at home and abroad are mainly focused on biomedical applications such as artificial organs, tissue engineering, and wound repair. [0003] Nanocellulose-based bioprinting inks that have been commercialized abroad mainly include Swedish nanocellulose-sodium alginate composite ink (CELLINK®). CELLINK® ink is a gel ink pr...

AI Technical Summary

Problems solved by technology

Although CELLINK® ink can smoothly 3D print different biomedical structures, 3D printing based on this composite ink still has a series of biocompatibility defects and deficiencies in the application of new biomedical materials:

[0004] ① Potential thermosensitizing impurities (endotoxin, (1,3)-beta-D-glucan) in the preparation process of nanofibers and sodium alginate have not been strictly controlled in accordance with relevant pharmacopoeias or medical clinical thresholds;

[0005] Sodium alginate and nanocellulose are both biologically inert, have no cell adhesion and cannot directly promote cell growth and reproduction, resulting in slow cell proliferation;

[0006] ③The mechanical strength of the printed structure is low or does not match the strength of the natural tissue. With the passage of time in cell culture or in vivo transplantation, the cross-linked calcium ions are lost, and the strength of the printed structure is weakened;

[0007] ④Because cellulose is difficult to effectively biodegrade in the human body, the biodegradation rate of the printed scaffold structure does not match the formation of new tissues;

[0008] ⑤ It is difficult for cells to effectively exchange nutrients and metabolic waste with the outside world in printed larger and thicker structures, resulting in reduced cell viability;

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