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Application of L-proline in increasing blastospore quantity and mycelial biomass of Cordyceps sinensis

A technology for Cordyceps sinensis fungus and blastospores, which is applied in the directions of application, plant cultivation, botanical equipment and methods, etc., can solve the problems such as excessive excavation of Cordyceps sinensis not being effectively curbed, shorten the time of artificial cultivation, reduce the cultivation cost, and improve the The effect of efficiency

Active Publication Date: 2019-07-19
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Cordyceps sinensis has been listed as a second-class protected species in the List of National Key Protected Wild Plants, the overharvesting of Cordyceps sinensis has not been effectively curbed

Method used

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  • Application of L-proline in increasing blastospore quantity and mycelial biomass of Cordyceps sinensis
  • Application of L-proline in increasing blastospore quantity and mycelial biomass of Cordyceps sinensis
  • Application of L-proline in increasing blastospore quantity and mycelial biomass of Cordyceps sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and add 1.5g of L-proline (final concentration is 10g / L, without L-proline as a control), and dilute to 150mL with water. After autoclaving, place in a 12±2°C culture room to pre-cool for later use.

[0029] Inoculate the inoculation mother liquor into the 500mL Erlenmeyer flask that 150mL Cordyceps sinensis liquid culture medium is housed, insert 1mL inoculation mother liquor to the final concentration of each bottle of culture medium to be 10 7 conidia / mL, wherein each bottle of culture medium contains about 15 blastospores, place the flask on a shaker with a rotation speed of 100 rpm, and cultivate it at 12±2°...

Embodiment 2

[0031] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and add 3g of L-proline (final concentration is 20g / L, without adding L-proline as a control), and dilute to 150mL with water. After autoclaving, place in a 12±2°C culture room to pre-cool for later use.

[0032] Inoculate the inoculation mother liquor into the 500mL Erlenmeyer flask that 150mL Cordyceps sinensis liquid culture medium is housed, insert 1mL inoculation mother liquor to the final concentration of each bottle of culture medium to be 10 7 conidia / mL, wherein each bottle of culture medium contains about 15 blastospores, place the flask on a shaker with a rotation speed of 100 rpm, and cultivate it at ...

Embodiment 3

[0034] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, add 0.38mg L-proline (final concentration is 2.5mg / L, no L-proline is used as a control), and dilute to 150mL with water. After autoclaving, place in a 12±2°C culture room to pre-cool for later use.

[0035] Inoculate the inoculation mother liquor into the 500mL Erlenmeyer flask that 150mL Cordyceps sinensis liquid culture medium is housed, insert 1mL inoculation mother liquor to the final concentration of each bottle of culture medium to be 10 7 conidia / mL, wherein each bottle of culture medium contains about 15 blastospores, place the flask on a shaker with a rotation speed of 100 rpm, and cultivate it at 12±2°...

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Abstract

The invention discloses application of L-proline in increasing blastospore quantity and mycelial biomass of Cordyceps sinensis. In order to solve the problem of how to increase mycelial biomass duringliquid fermentation of Cordyceps sinensis and the problem of how to acquire massive infection blastospores during artificial cultivation of entomogenous fungi with Cordyceps sinensis, L-proline is added to a Cordyceps sinensis medium to induce Cordyceps sinensis to efficiently produce blastospores; after Cordyceps sinensis spores are induced to the medium with the L-proline and cultured for 30 days, the quantity of blastospores is 1.49-5.26 times as high as that of a group with no addition; after 45 days of cultivation, the quantity of the blastospores is increased by 17-52.6 times; after 60days of cultivation, mycelial dry weight is increased by 15.67-66.44%. The blastospores for infecting Hepialus armoricanus so as to artificially cultivate entomogenous fungi with Cordyceps sinensis can be acquired more efficiently herein; the efficiency of acquiring mycelia by liquid fermentation of Cordyceps sinensis can be improved; artificial cultivation time of Cordyceps sinensis can be shortened; cultivation cost can be reduced.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of fungal culture, and in particular relates to the application of L-proline in increasing the number of blastospores and mycelia biomass of Cordyceps sinensis. [0003] Background technique: [0004] Cordyceps sinensis Ophiocordyceps sinensis is a traditional precious Chinese herbal medicine in my country. Together with ginseng and antler, it is known as the three treasures of Chinese medicine. Cordyceps sinensis is a complex of dead insects and fungal sporosomes formed by infecting the larvae of the family Hepialidae by Cordyceps sinensis fungus (also known as Hepialidae sinensis). Cordyceps sinensis has a special habitat (it only reproduces naturally in the high-altitude alpine meadow environment), with slow growth rate, long cycle and extremely limited natural resources. Although Cordyceps sinensis has been listed as a second-class protected species in the "List of National Key Protected Wild Pla...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 刘桂清曹莉韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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