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Method for separating and purifying lactic acid from lactic acid fermentation broth

A technology of lactic acid fermentation and lactic acid, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of increasing the cost of waste treatment, affecting the purity, and the low crystallization rate of calcium lactate, so as to reduce the discharge of waste liquid and reduce the The effect of extracting costs

Active Publication Date: 2019-07-02
GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] This technological process has the characteristics of simple equipment requirements and low cost, but there are certain deficiencies, such as converting lactic acid into calcium lactate in the process, but due to the influence of impurities such as sugars, proteins and pigments in the fermentation broth, the crystallization rate of calcium lactate is not high. High, so multiple crystallizations are required, which not only affects its purity, but also increases the time cost; anion and cation exchange resins are used in the process, and in the process of activation and regeneration of the resin, it takes a lot of acid, alkali and time. Produces a large amount of waste liquid, increasing the cost of waste disposal

Method used

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  • Method for separating and purifying lactic acid from lactic acid fermentation broth
  • Method for separating and purifying lactic acid from lactic acid fermentation broth

Examples

Experimental program
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Effect test

Embodiment 1

[0060] The preparation of above-mentioned lactic acid fermentation liquid, step is as follows:

[0061] ① Activated strains: Take the above-mentioned Lactobacillus sake preservation tube out of the -80°C low-temperature refrigerator, streak the single bacteria on the MRS plate with a sterile inoculation loop, and culture in a 30°C incubator for 18 hours;

[0062] ②Cultivate the monobacteria in MRS broth at 30°C for 12 hours;

[0063] ③Inoculate lactic acid bacteria into 1L of MRS broth according to the inoculum amount of 2%, and culture at 30°C for 36 hours;

[0064] ④ Detection of lactic acid: L-lactic acid, the concentration is 56.50mg / mL, and the total mass is 56.50g.

[0065] The separation and purification of lactic acid in lactic acid fermentation broth is as follows:

[0066] (1) heat treatment and removal of insoluble impurities such as bacteria:

[0067] Heat the fermentation broth to 70°C, stir at constant temperature for 9 minutes, and then centrifuge (rotating a...

Embodiment 2

[0083] The preparation of the above-mentioned lactic acid fermentation broth is the same as in Example 1.

[0084] The separation and purification steps of lactic acid in lactic acid fermentation broth are as follows:

[0085] (1) heat treatment and removal of insoluble impurities such as bacteria:

[0086] Heat the fermentation broth to 90°C, stir at a constant temperature for 8 minutes, and then centrifuge (9000 g for 15 minutes) to remove insoluble impurities such as bacteria and residual calcium carbonate, and filter the supernatant to obtain filtrate 1;

[0087] (2) Organic reagent precipitation to remove fat-soluble impurities:

[0088] ①Add 50% sulfuric acid to the filtrate 1 until the pH is 1.0, so that all the calcium lactate in the filtrate 1 is converted into lactic acid, concentrate by rotary evaporation at 70°C to a thick paste, and add ethanol to the concentrated solution (final concentration is 75%) , mix well, stand at 4°C for 4h, centrifuge (rotating speed i...

Embodiment 3

[0102] The preparation of the above-mentioned lactic acid fermentation broth is the same as in Example 1.

[0103] The separation and purification steps of lactic acid in lactic acid fermentation broth are as follows:

[0104] (1) heat treatment and removal of insoluble impurities such as bacteria:

[0105] Heat the fermentation broth to 85°C, stir at a constant temperature for 5 minutes, and then centrifuge (10000 g for 10 minutes) to remove insoluble impurities such as bacteria and residual calcium carbonate, and filter the supernatant to obtain filtrate 1;

[0106] (2) Organic reagent precipitation to remove fat-soluble impurities:

[0107] ① Add 60% sulfuric acid to the filtrate 1 until the pH is 1.0, so that all the calcium lactate in the filtrate 1 is converted into lactic acid, concentrate by rotary evaporation at 70°C to a thick paste, and add ethanol to the concentrated solution (final concentration is 60%) , mix well, stand at 4°C for 8 hours, centrifuge (rotating ...

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Abstract

The invention relates to the field of microbial separation and purification, in particular to a method for separating and purifying lactic acid from a lactic acid fermentation broth. The method mainlycomprises the following steps: performing heat treatment on the lactic acid fermentation broth, removing insoluble impurities such as thallus and the like; performing acid treatment; performing concentration; performing organic reagent precipitation; performing centrifugal filtration; performing concentration; performing decoloration; performing silica gel chromatography column; performing ODS column; and performing concentration to obtain the finished product. According to the method, the lactic acid finished product is obtained, the purity of the product is 93% or more, and the recovery rate of lactic acid is 67% or more. In the preparation process, the loss of main components is reduced, the material is saved, the discharge of waste liquid is reduced, and the treatment time is shortened.

Description

technical field [0001] The invention relates to the field of microorganism separation and purification, in particular to a method for separating and purifying lactic acid from lactic acid fermentation liquid. Background technique [0002] L-lactic acid, which is widely used in the food processing and medical industries, is usually produced by fermentation. Over the past decade, potential productivity has been gaining more and more attention due to the increase in its actual demand. Lactic acid (2-hydroxypropionic acid) is a naturally occurring hydroxycarboxylic acid first refined from yogurt by the Swedish chemist Scheele in 1780. Later, lactic acid gained importance in the food, pharmaceutical, cosmetic and other chemical industries due to its various uses as a sour agent, flavor enhancer and preservative. In addition, L-lactic acid can be used as a raw material for the production of polylactic acid (PLA), a polymer present in medical applications and an environmentally f...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C59/08
CPCC07C51/42C07C59/08
Inventor 刘陈立刘复荣蒙海林何敬愉崔金明刘明珠
Owner GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
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