Preparation method and application of high-efficiency aerobic denitrification phosphorus-accumulating organism immobilized spheres

Abstract

The invention discloses a preparation method and application of high-efficiency aerobic denitrification phosphorus-accumulating organism (DPAO) immobilized globules. The method comprises the followingsteps: 1) employing high-efficiency aerobic DPAO, named as Acinetobacter sp., belonging to acinetobacter, and having the preservation number of CGMCC No.17456; 2) selecting aerobic DPAO with preserved slopes, inoculating the aerobic DPAO into a liquid LB culture medium, and carrying out shake cultivation to obtain a bacterial suspension; and 3) embedding the bacterial suspension with a sodium alginate solution to obtain the immobilized spheres. The immobilized spheres are added into domestic sewage according to the adding amount of 20-60 g / l for nitrogen and phosphorus removal treatment, thenitrogen removal rate exceeds 80%, and the phosphorus removal rate exceeds 90%. The object for embedding the spheres is a single strain, and the embedding material is only sodium alginate. According to the process, nitrification liquid backflow is unnecessary, synchronous nitrogen and phosphorus removal can be achieved, the treatment effect is high, the operation process is simple, the productioncost is low, and popularization is easy.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment, and in particular relates to a technology for synchronous denitrification and phosphorus removal of waste water by immobilized pellets embedding a single bacterium. Background technique [0002] With the attention and great attention to the increasingly serious problem of water pollution at home and abroad, the state and local governments have promulgated and implemented new water pollutant discharge standards, and the control of the effluent indicators of sewage treatment plants is becoming more and more strict, especially for total phosphorus and total nitrogen. However, the existing sewage treatment plants in my country generally implement the "Pollutant Discharge Standards for Urban Sewage Treatment Plants" (GB18918-2002) Class A Standard, which means that the nitrogen and phosphorus removal effects of the wastewater treatment process must be improved, that is, the standard is up...

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Problems solved by technology

[0046] Among the existing invention patents, there are patents for immobilized microorganisms to denitrify and dephosphorize sewage, such as patents CN101259983A and CN103013974A, but because algae are autotrophic organisms, the application of nitrogen and dephosphorization in sewage requires light conditions and The low-carbon environment limits its application in the upgrading of sewage treatment plants

[0047] There are patents for immobilized microorganisms to denitrify and dephosphorize sewage, such as patents CN102392011A and CN108359663A, which embed a single bacterium, which can only denitrify or dephosphorize, but cannot achieve simultaneous denitrification and phosphorus removal. For example, adding two kinds of bacteria separately When bacteria cell pellets are used for denitrification and phosphorus removal, the operating conditions are inconsistent, which increases the cost of making pellets and sewage system operation

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