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A kind of phthalate and/or antibiotic pollution microbial remediation preparation and its preparation and application

A technology of phthalates and polluting microorganisms, applied in the field of biological treatment of environmental pollutants, can solve problems such as being difficult to adapt to the environment, unable to obtain a repair effect, etc., and achieve the effects of low price, convenient transportation and storage, and simple and convenient production process.

Active Publication Date: 2021-07-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of environmental conditions, when free microorganisms are added to the actual water body, there may be vicious competition with the microorganisms in the water environment or it is difficult to adapt to the environment, resulting in the failure to achieve good repair effects.

Method used

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  • A kind of phthalate and/or antibiotic pollution microbial remediation preparation and its preparation and application
  • A kind of phthalate and/or antibiotic pollution microbial remediation preparation and its preparation and application
  • A kind of phthalate and/or antibiotic pollution microbial remediation preparation and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation method for microbial restoration preparation, comprising the following steps:

[0047] 1) Mycobacterium (Mycobacterium) ASW6D strain is inoculated (inoculum amount is 2% culture medium amount) in inorganic culture medium (inorganic culture medium contains DEHP and SMT, DEHP and SMT are respectively 20mg / L and 5mg / L) , cultivated at 25-30°C for 36 hours, and continuously acclimatized 3 times; then inserted into a 500mL Erlenmeyer flask (the culture medium in the Erlenmeyer flask was an inorganic salt medium), and cultivated at 25-30°C and 150r / min for 12h; then press 10 The % inoculum size was inserted into a 5L seed tank (the culture medium in the tank was a nutrient gravy medium), and cultivated for 18 hours at 200r / min, pH7.0~7.5, and ventilation rate 3L / min to obtain a bacterial liquid; bacterial liquid Concentration: OD600=0.8~1;

[0048] The composition of the inorganic salt medium is: NaCl 1g, NH 4 NO 3 0.5g, FeCl 3 0.01g, K 2 HPO 4 0.76g, MgSO...

Embodiment 2

[0054] The microbial remediation preparation prepared in Example 1 was inoculated onto the nutrient gravy medium (the mass volume ratio of the microbial remediation preparation to the medium was 10g:100mL), in a shaker at 30°C, protected from light under the condition of 150r / min After 24 hours of shaking culture, the number of colonies was measured by the dilution coating plate method, reaching 5-7×10 8 pcs / g.

Embodiment 3

[0056] The microbial restoration preparation prepared in Example 1 is added to the MSM culture fluid containing DEHP of 20 mg / L and SMT of 5 mg / L, and the mass volume ratio of the microbial restoration preparation to the culture fluid is 2.5 g: 100 ml; Sterile granules and free bacteria (refers to the expanded culture of ASW6D bacteria, that is, bacterial liquid) as the control group, and a single MSM culture solution containing target pollutants as the blank group; The concentrations of pollutants (DEHP and SMT) in the culture medium were measured after cultured in the dark with shaking for 4 days.

[0057] figure 1 For the microbial repair preparation prepared in Example 1, the removal effect figure of sulfamethazine (SMT) at different times; wherein the microbial repair preparation prepared by immobilized bacterial agent-embodiment 1, immobilized sterile pellets represent no Bacteria solution, a pellet prepared by replacing sterile water, free bacteria means that mycobacte...

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Abstract

The invention belongs to the technical field of environmental pollutant treatment, and discloses a phthalate and / or antibiotic-contaminated microbial remediation preparation and its preparation and application. The microbial remediation preparation is mainly prepared from the following components: phthalate-degrading bacteria mycobacteria, biochar, biosurfactant, bioprotective agent, and bionutrients; mycobacteria are used in the form of bacterial liquid, The mycobacterium is a phthalate compound-degrading bacterium; the biochar is obtained by sintering corn stalks in an oxygen-free atmosphere; the restoration agent also includes a curing agent, which is obtained by curing each component. The invention also discloses a preparation method of the microorganism restoration preparation. The microbial remediation formulation is used to remove phthalates and / or antibiotics from water. The microbial remediation preparation of the present invention can quickly and efficiently remove bis(2-ethylhexyl) phthalate and sulfamethazine in wastewater, and has good effect.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a phthalate and / or antibiotic-contaminated microbial remediation preparation and a preparation method and application thereof. Background technique [0002] With the rapid growth of my country's economy and the obvious improvement of the level of urbanization, the contradiction with the water environment has become increasingly prominent, and the overall deterioration of my country's water bodies is still not optimistic. In particular, persistent organic pollutants represented by phthalates and antibiotics have been detected in all major water bodies, and the pollution of the two in the urban water environment is more serious. On the one hand, this has brought a great load to the urban sewage treatment plant, on the other hand, there are potential risks to the ecological environment and human health. [0003] As a commonly use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F101/34C02F101/30
Inventor 杨琛郭楚玲党志张政芳杨婧杨晶晶柳彦俊
Owner SOUTH CHINA UNIV OF TECH
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