Method for quickly propagating seedlings of dendrobium officinale Kimura et Migo
A technology for Dendrobium officinale and seedlings, which is applied in the field of rapid propagation of Dendrobium officinale seedlings, can solve the problems of high seedling production costs, traits and genetic variation of tissue culture seedlings, waste of manpower, financial and material resources, etc., to improve seed germination rate and protocorm differentiation rate, eliminating slow seedling stage, and reducing labor costs
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Embodiment 1
[0043] Embodiment 1, the seedling of Dendrobium candidum seedling without hormone one-step seedling rapid propagation technology
[0044] (1) Prepare the mature Dendrobium officinale capsule
[0045] In mid-April, three-year-old Dendrobium officinale flowering plants with purple stalks and soft feet were selected in the planting area of Dendrobium candidum for artificial cross-pollination; Mature capsules, in November, were picked from the germplasm nursery of Dendrobium candidum and stored in a 4°C refrigerator for later use. Such as figure 1 Shown is the microscopic state of mature Dendrobium officinale seeds.
[0046] (2) Prepare a conventional tissue culture room
[0047] It can be used in most of the tissue culture factories now, and the culture conditions are constant temperature: 23-25°C, humidity 40%-70%, light intensity 1500-2000LX, and light for 12 hours a day.
[0048] (3) Making seed suspension
[0049] Soak the capsules of Dendrobium officinale in 75% alcoh...
Embodiment 2
[0059] When aseptic sowing, the method of uniform sowing is adopted. Specifically, after sowing the Dendrobium officinale seed suspension into the hormone-free medium with a pipette gun, the culture bottle is evenly shaken, so that the Dendrobium officinale seeds are more evenly distributed on the surface of the medium. Others are the same as embodiment 1.
[0060] Such as figure 2 Shown, for embodiment 1 Dendrobium officinale seed sowing in the culture medium, the microscopic state of seed turning green after three weeks.
[0061] Such as image 3 As shown, the seeds of Dendrobium candidum in Example 1 were sown into the culture medium, and the microscopic state of the protocorm formed after the seeds germinated two months later, and the protocorm had differentiated into obvious leaf buds.
[0062] Such as Figure 4 Shown, be the protocorm that the density of the seed (greater than 200 grains / bottle) of industrialization dendrobium candidum seed directly sows on the cultu...
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