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Method for quickly propagating seedlings of dendrobium officinale Kimura et Migo

A technology for Dendrobium officinale and seedlings, which is applied in the field of rapid propagation of Dendrobium officinale seedlings, can solve the problems of high seedling production costs, traits and genetic variation of tissue culture seedlings, waste of manpower, financial and material resources, etc., to improve seed germination rate and protocorm differentiation rate, eliminating slow seedling stage, and reducing labor costs

Active Publication Date: 2019-06-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this process needs to be transferred three times, and the steps are still cumbersome. Multiple bottle changes and transfers not only waste manpower, financial resources, and material resources, but also increase the pollution rate of tissue culture seedlings, resulting in a decrease in the rate of dendrobium candidum tissue culture seedlings coming out of bottles. Reduced, resulting in high seedling production costs
Moreover, the addition of hormones in the medium has certain effects on the growth of Dendrobium candidum tissue culture seedlings, and improper hormone addition may cause the traits and genetic variation of tissue culture seedlings.

Method used

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  • Method for quickly propagating seedlings of dendrobium officinale Kimura et Migo
  • Method for quickly propagating seedlings of dendrobium officinale Kimura et Migo
  • Method for quickly propagating seedlings of dendrobium officinale Kimura et Migo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the seedling of Dendrobium candidum seedling without hormone one-step seedling rapid propagation technology

[0044] (1) Prepare the mature Dendrobium officinale capsule

[0045] In mid-April, three-year-old Dendrobium officinale flowering plants with purple stalks and soft feet were selected in the planting area of ​​Dendrobium candidum for artificial cross-pollination; Mature capsules, in November, were picked from the germplasm nursery of Dendrobium candidum and stored in a 4°C refrigerator for later use. Such as figure 1 Shown is the microscopic state of mature Dendrobium officinale seeds.

[0046] (2) Prepare a conventional tissue culture room

[0047] It can be used in most of the tissue culture factories now, and the culture conditions are constant temperature: 23-25°C, humidity 40%-70%, light intensity 1500-2000LX, and light for 12 hours a day.

[0048] (3) Making seed suspension

[0049] Soak the capsules of Dendrobium officinale in 75% alcoh...

Embodiment 2

[0059] When aseptic sowing, the method of uniform sowing is adopted. Specifically, after sowing the Dendrobium officinale seed suspension into the hormone-free medium with a pipette gun, the culture bottle is evenly shaken, so that the Dendrobium officinale seeds are more evenly distributed on the surface of the medium. Others are the same as embodiment 1.

[0060] Such as figure 2 Shown, for embodiment 1 Dendrobium officinale seed sowing in the culture medium, the microscopic state of seed turning green after three weeks.

[0061] Such as image 3 As shown, the seeds of Dendrobium candidum in Example 1 were sown into the culture medium, and the microscopic state of the protocorm formed after the seeds germinated two months later, and the protocorm had differentiated into obvious leaf buds.

[0062] Such as Figure 4 Shown, be the protocorm that the density of the seed (greater than 200 grains / bottle) of industrialization dendrobium candidum seed directly sows on the cultu...

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Abstract

The invention discloses a method for quickly propagating seedlings of dendrobium officinale Kimura et Migo. The method comprises the following steps that ripe capsules of the dendrobium officinale Kimura et Migo are utilized for preparing a seed suspension liquid, dibble seedling is conducted, and then one-step seedling growing culture is conducted. By means of the method, the purpose that the seeds of the dendrobium officinale Kimura et Migo are directly differentiated, grown and developed into robust seedlings with the length of 8-10 cm and with roots, stems and leaves in an organic matter culture medium in which no hormone is added without transfer. The manual transfer frequency in the seedling growing process is removed, the manual cost and tissue culture seedling contamination rate which is caused by transfer are reduced, the seedling survival period brought by seedling hurting due to transfer is removed, thus the growth period of tissue culture seedlings of the dendrobium officinale Kimura et Migo is shortened, the production steps of the seedlings of the dendrobium officinale Kimura et Migo are simplified to a great extent, and the production cost is reduced; moreover, heritable variation of the tissue culture seedlings of the dendrobium officinale Kimura et Migo caused by hormone addition in the tissue culture process can be avoided, the original characters of the seedlings are kept to the greatest extent, and the method can serve as an important method for production of the tissue culture seedlings of the dendrobium officinale Kimura et Migo.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for rapidly propagating dendrobium officinale seedlings. Background technique [0002] Dendrobium officinale Kimura et Migo is a herbaceous plant of the genus Dendrobium orchidaceae. Because the seeds are extremely small, the embryos are not fully developed, and there is no endosperm tissue, the germination rate is extremely low in the natural state, and the natural reproductive ability is also weak. Coupled with the increasingly severe damage to the ecological environment, wild Dendrobium candidum resources are on the verge of extinction, and have now been listed as one of the key protected medicinal plants by the state. In order to protect this endangered precious traditional Chinese medicine, researchers at home and abroad have carried out tissue culture and rapid propagation of Dendrobium officinale, seed germination and mycorrhizal propagation research, and have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 向增旭梁晖辉
Owner NANJING AGRICULTURAL UNIVERSITY
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