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Compound enzyme, preparation method thereof and application of compound enzyme

A compound enzyme and extraction method technology, which is applied in the direction of biochemical equipment and methods, enzymes, lyases, etc., can solve the problems of low extraction yield, high cost, impact on human body and environment, etc., and achieve improved yield and improved efficiency. Activity, the effect of reducing the viscosity of the material

Inactive Publication Date: 2019-06-21
宁夏夏盛实业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the embodiment of the present invention provides a compound enzyme to solve the problem of low yield, high cost and damage to the human body and the environment in the extraction of active ingredients in Senecio by water decoction or ethanol extraction in the prior art. problem of influence

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Compound enzyme

[0029] Above-mentioned composite enzyme comprises the following components by weight:

[0030] 14 parts of cellobiase, 7 parts of xylanase, 9 parts of cellulase, 11 parts of pectinase, 11 parts of glycerin, 6 parts of sodium chloride and 40 parts of water.

[0031] 2. Preparation method

[0032] The above-mentioned complex enzyme is prepared by the following method:

[0033] Weigh each component, mix each component at room temperature, and stir at 300 r / min for 25 min to obtain the complex enzyme.

Embodiment 2

[0035] 1. Compound enzyme

[0036] Above-mentioned composite enzyme comprises the following components by weight:

[0037] 16 parts of cellobiase, 5 parts of xylanase, 11 parts of cellulase, 9 parts of pectinase, 9 parts of glycerin, 4 parts of sodium chloride and 46 parts of water.

[0038] 2. Preparation method

[0039] The above-mentioned complex enzyme is prepared by the following method:

[0040] Weigh each component, mix each component at room temperature, and stir at 200 r / min for 35 min to obtain the complex enzyme.

Embodiment 3

[0042] 1. Compound enzyme

[0043] Above-mentioned composite enzyme comprises the following components by weight:

[0044] 15 parts of cellobiase, 6 parts of xylanase, 10 parts of cellulase, 10 parts of pectinase, 10 parts of glycerin, 5 parts of sodium chloride and 44 parts of water.

[0045] 2. Preparation method

[0046] The above-mentioned complex enzyme is prepared by the following method:

[0047] Weigh each component, mix the components at room temperature, and stir at 250 r / min for 30 min to obtain the complex enzyme.

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Abstract

An embodiment of the invention discloses compound enzyme, a preparation method thereof and an application of the compound enzyme. The compound enzyme comprises, by weight, 12-18 parts of cellobiase, 4-8 parts of xylanase, 8-12 parts of cellulose, 8-12 parts of pectinase, 8-12 parts of glycerin, 3-7 parts of sodium chloride and 38-50 parts of water. The compound enzyme can crack cellulose, hemi-cellulose and cell walls of groundsel, release of effective components of the groundsel is facilitated, viscous materials such as xylan, beta-glucan and pectin are hydrolyzed, viscosity of the materialsis reduced, effective components are separated out, settling time is shortened, and the yield of the effective components of the groundsel is effectively improved.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of enzyme preparations, in particular to a compound enzyme and its preparation method and application. Background technique [0002] Qianliguang, also known as Jiuliming, Jiuliguang, Qianliji, has bright eyes, Huanghuacao Huanghuamu, Jiulongguang and Jiulingguang. It is an herbaceous plant of Compositae, which grows in hillsides, under sparse forests, forest edges, roadsides, and ditch grass. Produced in Henan, Shaanxi, Jiangsu, Zhejiang, Guangxi, Sichuan. It is cold in nature, bitter in taste, and has the effects of clearing heat and detoxifying, improving eyesight, and relieving itching. It is mostly used for wind-heat and cold, red eyes, swelling and pain, diarrhea and dysentery, skin eczema and boils. [0003] Senecio is a traditional Chinese medicinal material, and the extraction method of effective components in Senecio is generally water decoction or ethanol extraction. At...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/18C12N9/26C12N9/88C12N9/24A61K36/28
Inventor 赵迎春李兵沈涛
Owner 宁夏夏盛实业集团有限公司
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