Automatic blood filtering dry photochemical method microchannel liver function three-item detection card

Abstract

The invention relates to a photochemical-transmission photometry method detection card capable of automatically filtering red blood cells and simultaneously detecting three indexes of a liver function: alanine transaminase (ALT), aspartate aminotransferase (AST) and glutamyl transpeptidase (gamma-GT or GGT) and belongs to the dry chemical analysis technology field. An automatic blood filtering dryphotochemical method microchannel liver function detection card is formed by five layers of film structures, and the structures contain holes and microchannels for loading, detecting and inlaying a blood filtering film. During a detection process, whole blood is filtered through the blood filtering film, and filtered serum enters into a detection hole through the microchannels. A detection item in a sample is quantitatively analyzed by testing the intensity changes of transmission light of the detection hole. By using the photochemical method microchannel liver function multi-item detection card, automatic blood filtering can be realized, the serum does not need to be separated, and the whole blood can be used to quickly and sensitively detect multiple biochemical indexes. A test result of the invention is linear, CV and other indexes can satisfy a clinical test requirement.

Description

Technical field: [0001] The invention relates to the field of dry chemical analysis, in particular to a photochemical transmission method microchannel and a detection card capable of simultaneously detecting multiple biochemical indicators. Background technique: [0002] γ-GT or GGT is a kind of phosphatase, and its distribution in liver disease is a common and extremely harmful disease. Its timely diagnosis and treatment are of great significance for the prevention and diagnosis of diseases. Transaminase is an indicator to reflect liver function, and alanine aminotransferase (ALT) is the most commonly used sensitive indicator. When 1% of liver cells undergo necrosis, the serum ALT level can be doubled; when the serum AST activity continues to increase beyond When ALT is active, it often indicates serious liver parenchyma damage, which is a sign of aggravated chronicity, especially when it is >2, it indicates that the epithelial layer of the bile duct in the liver is main...

AI Technical Summary

Problems solved by technology

[0005] (1) Dry chemical test strips, compared with traditional methods, are simple and fast, and can save costs, but the joint inspection of multiple items is limited

[0006] (2) Dry chemical detection board: Although the functions are relatively comprehensive and the detection efficiency is higher than that of dry chemical test strips, the process is too complicated and the cost is high

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