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Extraction method of taurine in crassostrea gigas

An extraction method, a technology of taurine, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of exposure and severe toxicity, and achieve reduction of energy consumption, increase of total yield and quality Effect

Inactive Publication Date: 2019-06-18
YANTAI APOLLO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome above-mentioned deficiencies in the prior art, and provide a kind of extracting method of taurine in the long oyster, mainly solve the raw material of existing taurine chemical synthesis method and all contact with highly toxic material issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Pre-treatment of raw materials, shelling oysters, washing away sediment and dirt with tap water;

[0022] 2) Homogenizing, the oyster meat is placed in a homogenizer to make slurry oyster meat;

[0023] 3) Boiling method extraction, pour the slurry oyster meat in step 2) into a stainless steel pot, add 3 times the volume of water and boil for 1 hour, separate the juice, add appropriate amount of water to the residue and boil for 0.5 hours, separate the juice, twice Juice merged;

[0024] 4) Precipitate the protein, concentrate the combined two juices to 1 / 6 of the original volume, add 1:1 (V:V) hydrochloric acid, adjust the pH to 3, that is, there is acidic protein precipitation, and separate by centrifugation to obtain the centrifugate, Use 5mol / L NaOH to adjust the pH of the centrifuge to 10, that is, the precipitation of basic protein, centrifuge, and adjust the pH of the centrifuge to 4-5 with hydrochloric acid.

[0025] 5) Ion exchange separation, the centrifu...

Embodiment 2

[0029] 1) Pre-treatment of raw materials, shelling oysters, washing away sediment and dirt with tap water;

[0030] 2) Homogenizing, the oyster meat is placed in a homogenizer to make slurry oyster meat;

[0031] 3) Boiling method extraction, pour the slurry oyster meat in step 2) into a stainless steel pot, add 3 times the volume of water and boil for 1 hour, separate the juice, add appropriate amount of water to the residue and boil for 0.5 hours, separate the juice, twice Juice merged;

[0032] 4) Precipitate protein, concentrate the combined two juices to 1 / 6 of the original volume, add 1:1 (V:V) hydrochloric acid, adjust the pH to 3, that is, acidic protein precipitation, use centrifugation (2000r / min) The centrifugal liquid is obtained by separation, and the pH of the centrifugal liquid is adjusted to 10 with 5 mol / L NaOH, that is, alkaline protein is precipitated, and the centrifugal separation is performed, and the pH of the centrifugal liquid is adjusted to 4-5 with ...

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Abstract

The invention relates to an extraction method of taurine in crassostrea gigas. The extraction method comprises the steps of performing coarse extraction through a water-boiling method by using the good water solubility of taurine; adjusting the pH to precipitate protein in a solution; removing impurities of strong electrolyte salt, polysaccharide and the like in the solution through a method of passing through a resin column, and finally performing further purification on taurine through crystallization and recrystallization. The extraction method of taurine in crassostrea gigas plays an important role in protecting ischemic brain cells, preventing cataract, lowering the blood glucose level, reducing blood fat, reducing blood pressure, preventing atherosclerosis and preventing diseases ofthe old.

Description

technical field [0001] The invention relates to the technical field of taurine extraction, in particular to a method for extracting taurine from oysters. Background technique [0002] There are two main methods for the production of taurine: chemical synthesis and natural extraction. At present, chemical synthesis is the most widely used method, but the raw materials and production process of chemical synthesis are exposed to highly toxic substances. With the improvement of people's living standards and the development of green chemistry, people are increasingly advocating the use of natural Extracted taurine. Therefore, it is necessary to find safe taurine resources. Taurine widely exists in many organisms except plants, especially in aquatic products, and exists in free form. The process of extracting taurine from seafood is simple and non-polluting, and the extraction of taurine from the leftovers of aquatic products has environmental protection significance and has a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C303/44C07C309/14
Inventor 于壮英
Owner YANTAI APOLLO BIOTECHNOLOGY CO LTD
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