The New Application of Polyacetylenic Compounds in Lowering Uric Acid
A compound and uric acid-lowering technology, which can be applied to active ingredients of hydroxyl compounds, medical preparations containing active ingredients, drug combinations, etc., can solve undisclosed problems
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Embodiment 1
[0044] Grind 30 kg of dried Codonopsis pilosula root, soak and extract at room temperature with 5 times the weight of 95% ethanol aqueous solution for 3 times, extract for one week each time, combine the extracts, distill and concentrate under reduced pressure until there is no alcohol smell, to obtain extract. The extract was dispersed in 1 volume of distilled water, and then extracted with ethyl acetate and n-butanol respectively to obtain 212 g of ethyl acetate extraction parts and 178 g of n-butanol extraction parts.
[0045] The ethyl acetate extraction part was separated by silica gel column chromatography (100-200 mesh), using dichloromethane-methanol as the eluent (volume ratio 50:1-50:30) for gradient elution, and the results were determined by TLC. Combined to obtain a total of five eluates from Fr.1.1 to Fr.1.5. The Fr.1.2 eluate was separated again by silica gel column chromatography, using dichloromethane-methanol as the eluent (volume ratio of 20:1-20:15) for gra...
Embodiment 2
[0055] 30 kg of dried Codonopsis cordifolioidea was soaked and extracted 3 times at room temperature with 95% ethanol solution with a volume concentration of 5 times the weight, and each time was extracted for 1 week. , get extract. The extract was dispersed in 1 volume of distilled water and extracted with n-butanol to obtain 151 g of n-butanol extraction fractions. The n-butanol extraction part is separated by AB-8 macroporous resin, and the gradient elution is carried out according to the following procedures: water, methanol aqueous solution with a volume concentration of 35%, methanol aqueous solution with a volume concentration of 75%, and methanol, and the mobile phases are collected separately The eluate was concentrated under reduced pressure to obtain 36 g of the elution fraction of methanol as the mobile phase. The elution site of methanol as mobile phase is separated by silica gel column chromatography (100-200 mesh), and the mixed solvent of dichloromethane-metha...
Embodiment 3
[0058] Get 100 kg of snow chrysanthemum medicinal material, soak and extract with 15 times the volume of ethanol aqueous solution with a volume concentration of 70% after pulverization, concentrate under reduced pressure to remove the organic solvent, extract the concentrated solution 3 times with 1 times the amount of ethyl acetate, combine the ethyl acetate layers, reduce Concentrate under reduced pressure to remove the organic solvent to obtain the ethyl acetate extraction fraction. The ethyl acetate extraction part was separated by ODS reversed-phase silica gel column chromatography, and the mixed solvent of methanol-water was used for gradient elution at 1mL / min according to the following procedure: the volume fraction of methanol was 10%, 30%, 45%, 60% %, 80%, 90%, and 100%, respectively, 6 eluates Fr.A-G were obtained.
[0059]Among them, Fr.B was separated by silica gel column chromatography, and the mixed solvent of ethyl acetate-ethanol was used as the mobile phase t...
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