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A kind of heterotrophic nitrifying-aerobic denitrifying bacteria and identification method thereof

An aerobic denitrifying bacteria and heterotrophic nitrification technology, applied in the field of microorganisms, can solve the problems of long reaction time, low denitrification efficiency and poor precipitation, and achieve the effects of fast growth rate, simple denitrification process and high cell yield.

Active Publication Date: 2021-04-13
杭州民安环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing biological denitrification wastewater treatment process, most of the denitrification bacteria are found to remove ammonia nitrogen, but the bacteria are easy to lose due to poor sedimentation, so the reaction time is long and the denitrification efficiency is low; while the mycelium of fungi has good sedimentation performance , easy to stay in the reactor, reducing the loss of bacteria, thereby improving its denitrification efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Pick a single colony of MA1 and inoculate it in LB medium. The composition of LB medium is: casein 10g / L, yeast extract powder 5g / L, NaCl 10g / L, the same below, 30°C, 180rpm shaker for 8h-10h Then inoculate the seed solution in 100mL glucose medium with a ratio of 0.5%. The glucose medium consists of: glucose 5g / L, ammonium chloride 0.382g / L, dipotassium hydrogen phosphate 0.147g / L, EDTA 0.0025g / L, ZnSO 4 ·7H 2 O 0.011g / L, MnSO 4 ·H 2 O 0.00154g / L, CuSO 4 ·5H 2 O0.000392g / L, Co(NO 3 ) 2 ·6H 2 O 0.00025g / L, Na 2 B 4 o 7 10H 2 O 0.000177g / L, CaCl 2 2H 2 O0.0667g / L, MgSO 4 0.289g / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.000185g / L, KOH 0.146g / L, nitrilotriacetic acid 0.2g / L, FeSO 4 ·7H 2 O 0.00698g / L, the same below; in 30 ℃, 180rpm shaker culture 24h; according to water quality ammonia nitrogen determination Nessler's reagent spectrophotometry HJ535-2009 measured the initial ammonia nitrogen of the medium is 100ppm, cultured after 24h bacterial agent treatment ...

Embodiment 2

[0044] Pick a single colony of MA1 and inoculate it in LB medium, shake it in a shaker at 30°C and 180rpm for 8h-10h, then inoculate the seed solution with 1% in 100mL glucose medium and shake it in a shaker at 30°C and 180rpm for 24h; according to the water quality Determination of ammonia nitrogen Nessler's reagent spectrophotometry HJ535-2009 measured the initial ammonia nitrogen of the medium as 100ppm, and after 24 hours of bacterial agent treatment, the ammonia nitrogen of the medium was 29.9ppm, and the removal rate was 70.1%.

Embodiment 3

[0046] Pick a single colony of MA1 and inoculate it in LB medium, shake it in a shaker at 30°C and 180rpm for 8h-10h, then inoculate the seed solution with 5% in 100mL glucose medium and shake it in a shaker at 30°C and 180rpm for 24h; according to the water quality Determination of ammonia nitrogen Nessler's reagent spectrophotometry HJ535-2009 measured the initial ammonia nitrogen of the medium as 100ppm, and after 24 hours of bacterial agent treatment, the ammonia nitrogen of the medium was 27.8ppm, and the removal rate was 72.2%.

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PUM

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Abstract

The invention discloses a heterotrophic nitrifying-aerobic denitrifying bacterium and an identification method thereof. The heterotrophic nitrifying-aerobic denitrifying bacterium is preserved in the China Typical Culture Collection Center, and the preservation number is: CCTCC NO:CCTCC M 2018289 , the strain was identified as Pichia jadinii by the 26S rDNA fragment identification method, named Pichia jadinii MA1; it has the performance of simultaneous aerobic nitrification, denitrification, and denitrification; it has fast growth rate, high cell yield, and requires The concentration of dissolved oxygen is low, the investment in cultivation is small, and the denitrification process is more simple and economical.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a heterotrophic nitrifying-aerobic denitrifying bacteria and an identification method thereof. Background technique [0002] Under human activities, water resources such as lakes and reservoirs in our country are polluted, and some even cannot be used as water supply resources. Reports in recent years have shown that surface water pollution across the country is serious, and they are all polluted by nitrogen compounds to varying degrees. These nitrogen pollutants are one of the main causes of water pollution and eutrophication in lakes and reservoirs, so the removal of nitrogen from water bodies is of great significance to purify water resources. [0003] Although the traditional denitrification process such as chemical method and physical chemical method can treat wastewater well, there are some defects, such as causing secondary pollution and high cost. Biodegradation is conside...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12Q1/6895C12Q1/6869C12Q1/04C02F3/02C02F3/34C12R1/84
CPCY02W10/10
Inventor 张碧军王成汪丽佳王伟王科
Owner 杭州民安环境工程有限公司
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