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Preparation method of globin peptide iron chelate

A technology of hemoglobin and chelate, which is applied in the field of preparation of organic trace element iron, can solve the problems that the preparation method of hemoglobin peptide iron chelate has not yet seen research reports and few developments, and achieves low price, The effect of high production efficiency and low cost

Inactive Publication Date: 2019-06-11
陈石良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are not many researches and developments on protein peptide iron chelates in China at present, and there are no research reports on the preparation method of hemoglobin peptide iron chelates.

Method used

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  • Preparation method of globin peptide iron chelate

Examples

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Effect test

Embodiment 1

[0022] (1) Preparation of pig blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tubular centrifuge to continuously centrifuge to obtain 450L of blood cells and 560L of plasma. ;

[0023] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 450L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 900L of hemolysis;

[0024] (3) Enzymolysis: Heating the hemolysis solution to 52°C while continuing to stir, adjusting the pH value to 8.0 with NaOH solution, then adding 2.0% protease by weight of the blood cell solution, stirring constantly, and detecting the enzymolysis solution at intervals of one hour pH value, when the pH value drops below 8.0, adjust the pH value to 8.0 with alkali supplementation solution, heat the enzymolysis solution for 8 hours, heat the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes, and obtain the enzymolysis...

Embodiment 2

[0030] (1) Preparation of pig blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tubular centrifuge to continuously centrifuge to obtain 438L of blood cells and 562L of plasma. Collect the blood cells, and spray dry the plasma to make plasma protein powder ;

[0031] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 440L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 870L of hemolysis;

[0032] (3) Enzymolysis: heat the hemolyzate to 55°C with continuous stirring, adjust its pH value to 8.5 with NaOH solution, then add protease at 2.5% of the weight of the blood cell solution, keep stirring, and check the enzymolysis solution at intervals of one hour pH value, when the pH value drops below 8.5, adjust the pH value to 8.5 with alkali supplementation solution, heat the enzymolysis solution for 6 hours, heat the enzymolysis solution to 85°C to inac...

Embodiment 3

[0038] (1) Preparation of pig blood cells: collect 1000L of fresh pig blood that has been anticoagulated, and use a tubular centrifuge to continuously centrifuge to obtain 430L of blood cells and 570L of plasma. ;

[0039] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 430L of deionized water, and stir continuously for 60 minutes to break the blood cells. The stirring speed is 30r / min to prepare 800L of hemolysis;

[0040] (3) Enzymolysis: Heat the hemolyzed solution to 50°C with continuous stirring, adjust its pH value to 9.0 with NaOH solution, then add protease at 3% by weight of the blood cell solution, keep stirring, and detect the enzymolyzed solution at intervals of one hour PH value, when the pH value drops below 9.0, adjust the PH value to 9.0 with alkali supplementation solution, heat the enzymolysis solution for 4 hours, heat the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes, and obtain the enzymolysis solution;

[00...

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Abstract

The invention relates to a preparation method of a globin peptide iron chelate. The preparation method comprises main steps of preparing porcine blood corpuscle liquid, performing haemolysis, performing enzymolysis, performing centrifuging, performing ultrafiltration, performing chelating, and performing spray drying so as to obtain the globin peptide iron chelate. According to the method disclosed by the invention, porcine blood which is low in price and broad in source is used as a protein peptide source, inorganic iron salt is used as an iron source, iron elements and protein peptide are chelated, the technological process is simple, the cost is low, and the method is suitable for large-scale industrial production. The prepared product namely the globin peptide iron chelate is high in content of small and medium molecule peptide, and high in the content of chelating-state iron ions, and is a novel efficient iron supplement for feeds.

Description

technical field [0001] The invention relates to a preparation method of organic trace element iron, in particular to a preparation method of hemoglobin peptide iron chelate. Background technique [0002] Iron is the trace element most needed by animals. Most of it exists in red blood cells in the form of hemoglobin, which is one of the constituents of body muscles and iron-containing enzymes. Iron is also a cofactor for superoxide dismutase, and iron is involved in the antioxidant action of cells. In the process of livestock and poultry production, iron deficiency anemia is the most common nutritional deficiency in animals. The lack of iron trace elements directly leads to slow growth and reduced feed conversion efficiency. At present, there are two types of iron supplements commonly used in the market: inorganic iron and organic iron. Inorganic iron ions tend to antagonize with some nutrients, and are easily affected by Ph value, lipids, protein, cellulose, oxalic acid, et...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 陈石良
Owner 陈石良
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