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Method for fermenting butanol by performing pretreatment on straw by adopting novel eutectic solvent

A deep eutectic solvent and butanol fermentation technology, applied in the field of bioengineering, can solve the problems of high cost, low glucose concentration, low hemicellulose removal rate, etc., and achieve the effect of less three wastes and low cost

Inactive Publication Date: 2019-06-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method also has high cost, and the removal rate of hemicellulose is still not high, so the final concentration of glucose cannot reach very high

Method used

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  • Method for fermenting butanol by performing pretreatment on straw by adopting novel eutectic solvent
  • Method for fermenting butanol by performing pretreatment on straw by adopting novel eutectic solvent
  • Method for fermenting butanol by performing pretreatment on straw by adopting novel eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Novel deep eutectic solvent (ethylamine hydrochloride: lactic acid) pretreatment rice straw

[0027] Take 100g of ethylamine hydrochloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor (see Table 1 for the molar ratio), add 10g of rice straw (the mass ratio of deep eutectic solvent to rice straw is 10:1), and heat at 140°C After stirring for 2 h, the mixture was centrifuged, and the precipitate was washed 3 times with deionized water. After drying to constant weight, sieve.

[0028] Add the treated straw to pH4.8, 50mM citric acid-sodium citrate buffer to form a mixed system, the final mass concentration of rice straw is 10% (w / v), add The cellulase was used for enzymolysis, and the enzymolysis was carried out at 50°C and 150rpm on a water bath shaker for 24h.

[0029] Table 1 Synthetic novel deep eutectic solvent method

[0030]

[0031] Simultaneously synthesize ionic liquids with different molar ratios of ethylamine hyd...

Embodiment 2

[0032] Embodiment 2: Novel deep eutectic solvent (ethylamine hydrochloride: lactic acid) combines sodium hypochlorite solution to pretreat rice straw

[0033] Take 100 g of ethylamine hydrochloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor (see Table 1 for the molar ratio and synthesis method), add 10 g of rice straw (the mass ratio of deep eutectic solvent to rice straw is 10:1), The mixture was stirred at 140° C. for 2 h, then the mixture was centrifuged, and the precipitate was washed 3 times with deionized water. After drying to constant weight, sieve. Then soaking with 0.5% sodium hypochlorite for 6 hours, drying and sieving to obtain the rice straw combined with the deep eutectic solvent and sodium hypochlorite. Add the treated straw to pH4.8, 50mM citric acid-sodium citrate buffer to form a mixed system, the final mass concentration of rice straw is 10% (w / v), add The cellulase was used for enzymolysis, and the enzymolysis was performed...

Embodiment 3

[0034] Example 3: Production of butanol by fermentation of pretreated rice straw hydrolyzate

[0035] Take a certain amount of combined pretreated rice straw, add pH 4.8, 50mM citric acid-sodium citrate buffer solution, and add The cellulase, so that the final solid-liquid ratio is 1:10, hydrolyzed on a water bath shaker at 50°C and 150rpm for 24h, centrifuged to obtain the rice straw hydrolyzate, and added the following ingredients to form a medium: corn steep liquor dry powder 10g L -1 , CaCO 3 4g·L-1 , (NH 4 ) 2 SO 4 2g·L -1 , K 2 HPO 4 0.5g·L -1 , MnSO 4 ·H 2 O 0.01g·L -1 . Adjust the pH to 6.5, and sterilize at 115°C for 20 minutes. Activated Clostridium saccharobutylicum DSM 13864 (10%, v / v) was inoculated and cultured anaerobically at 37°C. The result of fermentation and cultivation for 72h is as follows: figure 2 Shown, wherein, butanol production is 10.16g L -1 , the yield is The space-time yield is 0.14g·L -1 h -1 .

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Abstract

The invention discloses a method for fermenting butanol by performing pretreatment on straw by adopting a novel eutectic solvent, and belongs to the technical field of bioengineering. According to themethod for fermenting the butanol by performing pretreatment on the straw by adopting the novel eutectic solvent, pretreatment is performed on straw by using a novel donor eutectic solvent which is synthesized by using lactic acid as a hydrogen bond donor and dthylamine hydrochloride as a hydrogen bond receptor; enzymatic hydrolysis is carried out by adding cellulase so as to obtain an enzymatichydrolyzate; and then, the enzymatic hydrolyzate is utilized as a carbon source for fermenting the butanol. During the process of performing the pretreatment on the rice straw, relatively high hemicellulose removal rate (73.2%) and lignin removal rate (55.7%) are achieved; and total sugar concentration after enzymatic hydrolysis can be up to 59 g / L, wherein the glucose concentration is 58.3 g.L<-1>. Compared with an existing method for fermenting the butanol by adopting a chloride choline : formic acid : acetic acid ionic-liquid (42 g / L), the method has relatively great improvement on the total sugar concentration. The whole processes have the advantages of being low in cost, few in waste gas, waste water and industrial residues and so on. In addition, the hydrolyzate of the straw after the pretreatment can be used for producing the butanol by performing fermentation.

Description

technical field [0001] The invention relates to a method for pretreating straw to ferment butanol by using a novel deep eutectic solvent, and belongs to the technical field of bioengineering. Background technique [0002] Today's world is increasingly demanding non-renewable energy sources such as oil, natural gas and coal. The oil crisis in the 1970s has aroused the general concern of countries all over the world on the problem of future energy shortage. Traditional energy sources, coal, oil and natural gas are all non-renewable energy sources, and the development of remaining reserves is becoming more and more difficult. Moreover, my country is a large agricultural country with abundant lignocellulose raw materials. Crop straw is rich in cellulose and hemicellulose, which is a valuable renewable resource. However, in my country, quite a lot of crop straw is discarded or burned. It not only causes waste of resources, but also easily causes environmental pollution. Under s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12P19/14C12R1/145
Inventor 贾鹏王显众倪晔许国超邢婉茹龚磊
Owner JIANGNAN UNIV
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