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Method for improving enzymatic hydrolysis efficiency of poplar wood

A poplar wood enzymatic and enzymatic hydrolysis technology, which is applied in the field of biomass pretreatment and enzymatic hydrolysis, can solve the problems of large amount of cellulase usage, low enzymatic hydrolysis efficiency, inhibition of cellulase hydrolysis ability, etc., and achieve stable hydrolysis ability , The process is simple, and the effect of reducing space hindrance

Active Publication Date: 2019-06-04
NORTHWEST A & F UNIV
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  • Abstract
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Problems solved by technology

[0012] The purpose of the present invention is to solve the disadvantages of large amount of cellulase used in the existing poplar hydrolysis process and low enzymatic hydrolysis efficiency, and provide a method for improving the enzymatic hydrolysis efficiency of acetic acid hydrogen peroxide pretreatment poplar wood
[0014] Based on the existing technology, it is impossible to know why there is still a large amount of cellulase used and low enzymatic hydrolysis efficiency after pretreatment of poplar wood with acetic acid hydrogen peroxide, which is promising in application prospects. The inventors conducted in-depth research on this and finally found that acetic acid hydrogen peroxide After pretreatment of poplar, there will be a certain amount of cellobiose accumulation in the process of enzymatic hydrolysis, thereby inhibiting the hydrolysis ability of cellulase, leading to the above problems

Method used

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Examples

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Embodiment 1

[0039] A method for improving the enzymatic hydrolysis efficiency of poplar wood includes the following steps:

[0040] Step 1) preprocessing

[0041] 1.1) Measure 60 mL of 99% pure acetic acid and 60 mL of 30% pure hydrogen peroxide, add a catalytic amount of 100 mmol / L sulfuric acid, and mix to prepare an acetic acid hydrogen peroxide pretreatment solution;

[0042] 1.2) Weigh 10 grams of the absolutely dry poplar wood residue pretreated with acetic acid and place it in a polytetrafluoroethylene lined steel cylinder. Add a volume concentration of 100% acetic acid and hydrogen peroxide solution according to the ratio of material to liquid ratio of 1:10. Pretreatment at 80°C for 2 hours; the above-mentioned acetic acid pretreatment refers to adding acetic acid with a purity of 5% to poplar wood and treating it at 170°C for 30 minutes;

[0043] 1.3) Cool down after pretreatment, use a centrifuge for solid-liquid separation, collect the filter residue, and wash it with water until it is...

Embodiment 2

[0055] The pretreatment method and reaction conditions are basically the same as those in Example 1. The difference is that the volume concentration of the acetic acid hydrogen peroxide solution in the acetic acid hydrogen peroxide pretreatment is 80%; if only 20FPU cellulase is added during hydrolysis, the calculated glucose yield is 67.2% The yield of xylose is 72.4%. After adding 500 nkat of β-glucosidase per gram of substrate, the yield of enzymatically hydrolyzed glucose increased to 91.1%, and the yield of xylose increased to 94.9%.

Embodiment 3

[0057] The pretreatment method and reaction conditions are basically the same as those in Example 1. The difference is that in the process of enzymatic hydrolysis, 1000nkat of β-glucosidase is added per gram of substrate. The final glucose yield is 97.5% and the xylose yield is 99.7%. .

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Abstract

The invention provides a method for improving enzymatic hydrolysis efficiency of poplar wood. Poplar wood pretreated with an acetic acid hydrogen peroxide solution is used as a raw material, and the problems of large usage quantity of cellulases and low enzymatic hydrolysis efficiency existing in an existing hydrolyzing process are solved. According to the method, based on the cellulases, beta-glucosidases is additionally added to an enzymatic hydrolysis reaction system, so that the highest glucose yield of the poplar wood can reach 99.4%, and the glucan in the poplar wood is nearly hydrolyzedcompletely. The method is high in efficiency, low in cost and simple and convenient to operate, complete enzymatic hydrolysis of the poplar wood is nearly realized, and a foundation is established for preparation of biological energy sources and chemicals through bioconversion of the poplar wood.

Description

Technical field [0001] The present invention relates to biomass pretreatment and enzymatic hydrolysis technology, in particular to a method for improving the enzymatic hydrolysis efficiency of poplar wood after acetic acid hydrogen peroxide pretreatment by adding β-glucosidase. Background technique [0002] Poplar is a fast-growing and high-yielding tree species that is planted in a large area in China. It grows fast, has abundant resources and high cellulose content. Therefore, biotransformation of poplar to produce bioenergy and chemicals has great potential. Pretreatment is an important step to improve the enzymatic hydrolysis efficiency of poplar wood. The pretreatment of poplar wood is mainly divided into methods such as acid, alkali, ionic liquid, and steam explosion. [0003] Huang et al. in 2018, J Wood Chem Technol.38(5):371–384 treated with 6.5% acetic acid at 172°C for 27 minutes, added 20FPU / g dry matter cellulase, the enzymatic hydrolysis yield of poplar wood 51%. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 张军华文沛瑶张甜贾丽丽
Owner NORTHWEST A & F UNIV
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