Hybridoma cell strain E secreting anti-glibenclamide monoclonal antibody and application thereof

Abstract

The invention provides a hybridoma cell strain E secreting an anti-glibenclamide monoclonal antibody and application thereof, and belongs to the field of food safety immunodetection. The hybridoma cell strain E secreting the anti-glibenclamide monoclonal antibody is deposited in CGMCC, the common microbiology center of the China Microbial Culture Collection Management Committee, and is classifiedas a monoclonal cell strain, the deposit date is May 24, 2018, and the deposit number is CGMCC No.17305. According to the hybridoma cell strain E, a complete antigen of glibenclamide and an equal amount of Freund's adjuvant are mixed and emulsified, and immunization BALB / c mice are subjected to subcutaneous multiple point injection atbacksand necks to finally obtain the hybridoma cell strain E secreting the anti-glibenclamide monoclonal antibody. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (IC50 value of 0.8 ng / mL) for the glibenclamide,can detect the residual amount of the glibenclamide in health foods, provides raw materials for the immunodetection of glibenclamide residues in the foods, and has a practical application value.

Description

technical field [0001] The invention relates to a hybridoma cell line No. E secreting anti-glibenclamide monoclonal antibody and its application, which belongs to the field of food safety immune detection. Background technique [0002] Glibenclamide (Glibenclamide) is a representative of the second generation of sulfonylurea oral hypoglycemic drugs. Its mechanism of action is mainly to promote the secretion of insulin by pancreatic islet B cells. Glycogenolysis and gluconeogenesis reduce liver production and output of glucose, and may also increase extrapancreatic tissue sensitivity to insulin and glucose utilization. Therefore, the overall effect is to reduce fasting blood glucose and postprandial blood glucose. Its curative effect is characterized by strong hypoglycemic effect and long duration. Due to the rapid growth rate of diabetic patients in our country, there are also a wide variety of diabetes non-prescription drugs and health food markets in our country. At pre...

AI Technical Summary

Problems solved by technology

However, the sample pretreatment process of these methods is complicated and the experimental cycle is long, which is not suitable for the rapid detection of a large number of samples.

However, the detection standard and determination method of glyburide have not been established in China, which brings great difficulties to the production and use of glibenclamide food additives, import and export trade, and food hygiene monitoring.

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