Integrated droplet microfluidic chip structure, preparation method and microfluidic chip assembly

Abstract

The invention provides an integrated droplet microfluidic chip structure. All functional modules of droplet generation, amplification and detection are integrated on the same microfluidic chip to achieve the whole enclosed process from the droplet generation to fluorescence detection. The invention also relates to an integrated droplet microfluidic chip structure preparation method and a microfluidic chip assembly. The structure is compatible with the positive pressure or negative pressure drive mode, the pressure response time is short, the rapid droplet generation can be achieved, and the sample preparation time is greatly reduced. Droplet generation oil is not required to be filled in advance, the operation is simple, and popularization and application in the technical field of digitalPCR are facilitated.

Description

technical field [0001] The invention relates to the technical field of PCR, in particular to an integrated droplet microfluidic chip structure and preparation method, and a microfluidic chip component. Background technique [0002] Polymerase chain reaction (Polymerase Chain Reaction, PCR) can realize the in vitro amplification of DNA sequence. It is the main method of nucleic acid detection at present, and it is also an important supporting technology in the field of life science research and clinical molecular diagnosis. It has greatly promoted the development of life science and other fields. rapid development of the field. At present, PCR technology has been developed to the third generation - digital PCR (Digital PCR, dPCR), the basic principle is to distribute the sample into a large number of reaction units, each unit contains one or more copies of the target molecule (DNA template), Perform PCR amplification on the target molecule, and perform statistical analysis o...

AI Technical Summary

Problems solved by technology

In this method, the air bubbles are distributed on the edge of the chamber by changing the height of the droplet collection chamber, and the air bubbles are not completely eliminated; and in the process of PCR amplification, the air bubbles easily lead to the fusion of droplets

In 2017, Jing Fengxiang and others made a glass-based droplet collection chamber. The operation process is cumbersome, and the effective integration of droplet generation and collection structures cannot be realized, and bubbles are easy to form

However, due to its unique droplet generation method, the pressurization and depressurization time of the system is longer than 30 minutes, which greatly increases the overall time of PCR detection and reduces the detection efficiency; The operation of the chip puts forward higher requirements and increases the difficulty of operation; in addition, the inherent characteristics of the chip structure design make the volume of the droplet uncontrollable, the detection throughput of the sample is limited, and the improvement space is limited

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