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Application of alpha-trithiophene in preparation of drugs for treating prostate cancer

A technology for prostate cancer and triple thiophene, which can be used in drug combinations, antineoplastic drugs, pharmaceutical formulations, etc., can solve the problems of increased family burden, poor treatment effect, and single treatment type of PCa patients

Inactive Publication Date: 2019-05-31
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most PCa medicines still rely on imports. Since foreign pharmaceutical companies are in a monopoly position in PCa medicines, the price of imported medicines is high, often costing more than 10,000 yuan. The high cost of medicines has increased the burden on the families of Chinese PCa patients.
The development of PCa drugs in China is late, and the technical level is far behind that of western developed countries. With the continuous advancement of technology, domestic PCa drugs have developed, and the average price is below 1,000 yuan, but the treatment effect is poor and the treatment type is single.

Method used

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  • Application of alpha-trithiophene in preparation of drugs for treating prostate cancer
  • Application of alpha-trithiophene in preparation of drugs for treating prostate cancer
  • Application of alpha-trithiophene in preparation of drugs for treating prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Effect of α-tertiary thiophene on the proliferation of prostate cancer cells

[0022] A. LNCaP cell culture

[0023] LNCaP cells were isolated and established by Horoszewicz JS in 1977 from the fine-needle aspiration tissue of the left supraclavicular lymph node of a 50-year-old Caucasian male patient definitely diagnosed with metastatic prostate cancer, and were purchased from the Chinese Academy of Sciences Stem Cell Bank. After thawing LNCaP cells, culture them in RPMI 1640 (w / o Hepes) containing 10% (v / v) fetal calf serum at 37°C, 5% CO 2 in the cell culture incubator. Before passaging, use an inverted microscope to carefully observe the growth of the cells attached to the wall. When the density of the adherent cells reaches about 80%, collect the cells for passaging or seeding the plate for experiments. 1:3 subculture, change the medium 2-3 times a week.

[0024] B. 22Rv1 cell culture

[0025] 22Rv1 is a human PCa epithelial cell line derived from xen...

Embodiment 2

[0034] Example 2 Effect of α-tertiary thiophene on the apoptosis of prostate cancer cells

[0035] LNCaP, 22Rv1, PC-3, and DU145 cells in the logarithmic growth phase were selected, digested with trypsin, and the cell concentration was adjusted to 3.0×10 5 / mL, inoculate the cells into a 6-well plate, add 1 mL of cell suspension to each well, and add 1 mL of complete culture medium. Then place at 37 °C, 5% CO 2cultured in an incubator. After the cells were completely adhered to the wall, thiophene compounds were added under the condition of turning off the fluorescent lamp (set 4 concentration gradients of 10 μM, 20 μM, 40 μM, and 80 μM, and each concentration was set with 3 replicate wells, and the experiment was repeated 3 times), and the blank control group was added with 1 ‰DMSO complete medium. Press eBioscience 48 hours after compound action TM Annexin V-FITC Apoptosis Detection Kit (Invitrogen, BMS500FI-300) instructions collected cells, prepared samples, and detect...

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Abstract

The invention discloses alpha-trithiophene which can inhibit the proliferation of prostate cancer cells. An experiment shows that alpha-trithiophene has a good inhibitory effect on different prostatecancer cells and can treat prostate cancer by inducing apoptosis of LNCaP cells, 22Rv1 cells, DU145 cells and PC3 cells. Moreover, as the alpha-trithiophene is derived from natural Chinese herbal extracts and low in production cost, toxic and side effect and risk, the alpha-trithiophene has great potential to the development of drugs for treating the prostate cancer, and a new drug choice can be provided for the treatment of prostate cancer.

Description

technical field [0001] The invention belongs to the technical field of prostate cancer treatment, and in particular relates to the application of alpha-tertiary thiophene in the preparation of drugs for treating prostate cancer. Background technique [0002] According to the 2018 global cancer statistics, prostate cancer (Prostate cancer, PCa) is the second most common cancer in men. With the aging population, the incidence of PCa in China is increasing year by year. However, due to the low rate of early diagnosis, the mortality rate of PCa patients in China is much higher than that in developed countries. In the United States, the survival rate of patients with the disease for 5 years is over 98%, while the survival rate of the same patients in China is only 69.2%. At present, most PCa medicines still rely on imports. Since foreign pharmaceutical companies are in a monopoly position in PCa medicines, the price of imported medicines is high, often costing more than 10,000 ...

Claims

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Application Information

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IPC IPC(8): A61K31/381A61P35/00A61P13/08
Inventor 王雪妮
Owner GUANGXI UNIV OF CHINESE MEDICINE
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