60Co-gamma ray radiation mutation breeding method for iris sanguinea tissue culture seedlings

Abstract

The invention relates to a 60Co-gamma ray radiation mutation breeding method for iris sanguinea tissue culture seedlings. The method comprises the following steps of 1, tissue culture seedling selection; 2, 60Co-gamma ray radiation treatment; 3, tissue culture seedling mutant primary selection; 4, enrichment subculture of primarily selected mutants; 5, rooting culture of primarily selected mutanttissue culture seedlings; 6, domestication transplanting; 7, field seedling mutant screening; 8, cutting propagation, tissue culture propagation and artificial selection. According to the method, theiris sanguinea tissue culture seedlings are adopted as materials, 60Co-gamma rays are utilized for mutation treatment, the mutagenic effect of different radiation dosages on the iris sanguinea tissueculture seedlings is observed, the proper radiation dosage is determined, and the iris sanguinea tissue culture seedling 60Co-gamma ray radiation mutation breeding technology is set up; by means of the method, rich heritable variations can be created, the breeding period of iris sanguinea is effectively shortened, a simple, convenient and efficient method is provided for new variety breeding, andthe hereditary basis of iris sanguine genetic resources is widened.

Description

technical field [0001] The present invention relates to a kind of Tissue culture seedling 60 Co-γ-ray radiation mutation breeding method. Background technique [0002] Iris sanguinea is a perennial herbaceous flower belonging to the family Iridaceae, with blue-purple and white flowers, black-brown reticular and yellow spots at the base of the outer perianth, and no appendages. It can grow well in wetlands and dry lands. It has strong disease resistance, cold resistance and moisture resistance, and has high ornamental value. It is an excellent material for garden wetland landscaping and creating natural wild interest. The breeding work of Iris plants in my country is progressing slowly, and the research on the breeding technology of Iris is of great significance for enriching the germplasm resources of Iris plants and developing and utilizing wetland plant resources. [0003] Radiation mutagenesis is mainly to treat materials with a certain dose of radiation, so as to cause...

AI Technical Summary

Problems solved by technology

[0005] There is a single breeding method for Iris plants, currently there are only conventional breeding techniques such as selfing and distant hybridization, and there is no report on the use of tissue culture seedlings of Iris to induce radiation and produce stable mutants.