Haworthia truncatapropagating method by test tube micro-cuttage
A technology of jade fan brocade and micro-cutting, which is applied in the direction of vegetative propagation, horticultural methods, botanical equipment and methods, etc., can solve the problems of high price, low survival rate of jade fan brocade leaf cuttings, high ornamental value, etc., and achieve stability , high economic value and practical effect
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[0029] (1) Selection and disinfection of materials Take the finished seedlings of Jade Shanjin as the material, put them in the greenhouse to control the water for 1 week, then pull them out, cut off the roots, rinse them, and then place them on the ultra-clean workbench with 75% alcohol Soak for 30s, then sterilize with 0.1% mercuric acid solution containing 1-2 drops of Tween-80 for 10 minutes, rinse with sterile water for 3 to 5 times, and filter the residual water with sterile filter paper;
[0030] (2) Under aseptic conditions, remove the leaves of the sterilized jade fan without brocade, and keep the integrity of the growth point of the base of the leaves with brocade during the grafting process. In order to increase the bud induction rate of Yufan leaves, the basic medium MS was improved, and the improvement method was as follows: reduce the amount of ammonium nitrate in MS, and increase the amount of potassium dihydrogen phosphate , added calcium nitrate. The improved...
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