A Method for Rapid Mass Production of Whole Female Loach Without Using Sex Hormone

A large-scale technology for female loach, applied in the field of rapid mass production of full-female loach without the use of sex hormones, can solve problems such as the negative impact of farmed fish or natural ecosystems, achieve good environmental benefits, reduce costs, and speed up production. Effect

Active Publication Date: 2021-04-13
HUAZHONG AGRI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Although individuals with sexual reversal induced by sex steroid hormones are only used as parents and will not enter the consumer market, the use of sex hormones (whether directly soaking fry, soaking live bait for fry, or feeding with mixed feed) will inevitably lead to the residue or metabolism of some sex hormones. The product enters the aquaculture water body or natural water body (pond, lake, reservoir, etc.), and has a negative impact on the aquaculture fish or the natural ecosystem

Method used

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  • A Method for Rapid Mass Production of Whole Female Loach Without Using Sex Hormone
  • A Method for Rapid Mass Production of Whole Female Loach Without Using Sex Hormone
  • A Method for Rapid Mass Production of Whole Female Loach Without Using Sex Hormone

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Embodiment 1

[0029]The present invention will be further described below in conjunction with the specific embodiments and the accompanying drawings to better understand the invention and will be implemented, but the experimental examples are not limited to the present invention.

[0030]Bonded belowfigure 1 withfigure 2The present invention is described in further detail, and a method of unused sex hormones is a large-scale production of full-female mud, and the steps are:

[0031](1) Artificial breeding and hatching: Choosing the gland's development of muddy females and male fish, demanding that the parental surface has no obvious trauma, no parasites attached, no appearance, no deformity, 50mg / L MS-222 before using 50mg / L MS-222 Anesthesia, using 5 ug / kglhrh-2,3 mg / kg / kglhrh-2,3mg / kg DOM and 1000Iu HCG three oxytoculations for primary injection, and the male dose is reduced. After the effect time (6-20 hours), mixed with 0.6% brine. Wet method is insemination, sticking the feeding eggs n...

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Abstract

The invention discloses a method for rapid and large-scale production of whole female loach without using sex hormones. The steps are: (1) Artificial reproduction and hatching: selecting gonad loach females and males, with no trauma or parasite attachment on the parent body surface , No appearance deformity, anesthetized before artificial oxytocin, artificial insemination after injection of oxytocic agent, naturally stick the fertilized eggs on the mesh, and transfer them to plastic boxes for artificial incubation; (2) Reversal of high temperature conductivity: use a heating rod to The speed of raising the culture water temperature gradually, continuous high-temperature treatment, and then cooling culture; (3) test cross and group expansion: the remaining male fish are cultured to sexual maturity, and the offspring produced by each group of male and female fish are divided into two parts and raised separately. Eliminate the female individuals in the group, and raise the remaining XX-type male fish to sexual maturity; (4) All female loach is produced. The method is easy to operate and easy to operate, and fast-growing all-female loaches can be rapidly produced without using sex steroid hormones in the whole process.

Description

Technical field[0001]The present invention belongs to the field of fish single-sex production technology or fish artificial gender control technology, and more particularly to a method of unused sex hormone rapid large-scale production of full female mud.Background technique[0002]Misgurnus Anguillicaudatus, 2017, the national output of 390,000 tons, which increased by about 6% from 2016. It is one of the fastest growing breeding varieties in freshwater fish. In 2017, the national freshwater fish production is ranked 11th (2018 China Fishery) Statistical Yearbook). my country's loach production is 99.7% of the world's output, 2016 World total output value of $ 943 million, accounting for the world's freshwater fish output value (Fisheries and Aquaculture Software, 2018, FAO), is an important economic fish in the world and my country.[0003]Under the same breeding conditions or experimental conditions, the muddy female and female age showed a significant growth difference, and the fema...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCY02A40/81
Inventor 沈志刚樊启学袁勇超王凌宇齐飘飘陈敏
Owner HUAZHONG AGRI UNIV
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