Serum-free culture medium capable of proliferating hematopoietic stem cells and preparation method of culture medium

A technology of serum-free medium and hematopoietic stem cells, applied in the field of serum-free medium and its preparation, can solve problems affecting the effect of hematopoietic stem cell transplantation, failure of hematopoietic stem cell transplantation, immune rejection of patients, etc., to maintain multi-directional differentiation ability, Guarantee the shape and multi-directional differentiation of cells, and enhance the effect of cell proliferation rate

Inactive Publication Date: 2019-05-10
广东美赛尔细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, animal-derived components often contain a certain amount of immunogenic components or pathogenic microorganisms. These foreign substances will cause severe immune rejection in patients, affect the effect of hematopoietic stem cell transplantation, and eventually lead to the failure of hematopoietic stem cell transplantation.

Method used

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  • Serum-free culture medium capable of proliferating hematopoietic stem cells and preparation method of culture medium
  • Serum-free culture medium capable of proliferating hematopoietic stem cells and preparation method of culture medium
  • Serum-free culture medium capable of proliferating hematopoietic stem cells and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, a kind of serum-free culture medium that can expand hematopoietic stem cell

[0033] The serum-free medium for expanding hematopoietic stem cells uses DMEM / F12 medium as a solvent, and consists of the following components and their concentrations:

[0034]

[0035] The preparation method is:

[0036] Take a pack of DMEM / F12 medium powder, add 900ml deionized water, then add 3g NaHCO 3 , add 3gHepes, stir for 1h, adjust the pH to 7.2-7.4, add the following ingredients stephaline, berberine, bFGF, aFGF, IGF1, IGF2, transferrin, insulin growth factor, EGF, interleukin 1, interleukin 2, interleukin 6. Dissolve stem cell factor, thrombopoietin and FMS-like tyrosine kinase 3 ligand evenly, dilute to 1L with deionized water, adjust the pH value to 7.2-7.4, and pass through a 0.22μm filter membrane at room temperature.

Embodiment 2

[0037] Embodiment 2, a kind of serum-free culture medium that can expand hematopoietic stem cells

[0038] The serum-free medium for expanding hematopoietic stem cells uses DMEM / F12 medium as a solvent, and consists of the following components and their concentrations:

[0039]

[0040] The preparation method is similar to Example 1.

Embodiment 3

[0041]Example 3, a serum-free medium capable of expanding hematopoietic stem cells

[0042] The serum-free medium for expanding hematopoietic stem cells uses DMEM / F12 medium as a solvent, and consists of the following components and their concentrations:

[0043]

[0044]

[0045] The preparation method is similar to Example 1.

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Abstract

The invention belongs to the field of traditional Chinese medicine detection and particularly relates to a serum-free culture medium capable of proliferating hematopoietic stem cells and a preparationmethod of the culture medium. The provided serum-free culture medium capable of proliferating hematopoietic stem cells comprises stephanine, berberine, bFGF, aFGF, IGF1, IGF2, transferrin, insulin growth factors, EGF, interleukin 1, interleukin 2, interleukin 6, stem cell factors, thrombopoietin, fMS-like tyrosine kinase 3 ligands and a DMEM / F12 culture medium. By adding stephanine and berberine,the serum-free culture medium not only has the capability of proliferating the hematopoietic stem cells derived from placentae, bone marrow and peripheral blood in vitro, improving the adherence capability of the hematopoietic stem cells and increasing the cell proliferation rate of the hematopoietic stem cells but also can maintain the self-renewal capability and multidirectional differentiationcapability of the stem cells. The hematopoietic stem cells proliferated by the serum-free culture medium have a good effect of promoting the reconstruction of a hematopoietic system in the body of apatient.

Description

technical field [0001] The invention belongs to the field of detection methods of traditional Chinese medicine compositions, and in particular relates to a serum-free culture medium capable of expanding hematopoietic stem cells and a preparation method thereof. Background technique [0002] Hematopoietic stem cells are the source of blood cells, have the ability of self-renewal and multi-directional differentiation, and are also an important component in the treatment of malignant blood diseases. They have important clinical and commercial value in the treatment of malignant leukemia and tumor diseases. The sources of hematopoietic stem cells include placenta, umbilical cord blood, peripheral blood and bone marrow. Peripheral blood and bone marrow are the main sources of hematopoietic stem cells for clinical transplantation at present, but they are likely to bring pain and other problems to healthy donors during the collection process. It's not necessarily smooth. In additi...

Claims

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Application Information

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IPC IPC(8): C12N5/0789
Inventor 徐单单梅建勋陈素红
Owner 广东美赛尔细胞生物科技有限公司
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