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Heparin detection kit and application thereof

A detection kit and detection reagent technology, applied in the field of anticoagulation effect detection, can solve the problems of lack of fibrinogen, fibrinolytic activity affecting the accuracy of detection results, etc., and achieve the effect of evaluating anticoagulation effect

Pending Publication Date: 2019-05-03
GUIZHOU JINJIU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, APTT (activated partial thrombin time) is mainly used to evaluate the anticoagulant effect of heparin clinically. The presence of heparin sodium salt in the blood will lead to prolonged APTT, but it is susceptible to congenital coagulation factors Ⅱ, Ⅴ, Ⅷ, and Ⅸ. , Ⅺ, Ⅻ decreased, lack of fibrinogen, enhanced fibrinolytic activity or other anticoagulant substances will affect the accuracy of the test results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment provides a preparation method of a heparin detection reagent, the preparation method comprising the following steps:

[0039] The heparin detection reagent includes: heparinase I, anhydrous calcium chloride, glycerol and D-trehalose; wherein, the heparinase I is in a liquid state.

[0040] Specifically, the concentration of heparanase I was 8 IU / mL, the concentration of anhydrous calcium chloride was 0.2%, the concentration of glycerol was 0.6%, and the concentration of D-trehalose was 0.6%.

Embodiment 2

[0042] This embodiment provides a preparation method of a heparin detection reagent, which is roughly the same as the method provided in Example 1, the difference lies in the different components, and the difference is as follows:

[0043] The heparin detection reagent includes: heparinase I, anhydrous calcium chloride, glycerol and D-trehalose according to the mass percentage; wherein, the heparinase I is in a liquid state.

[0044] Specifically, the concentration of heparanase I was 7 IU / mL, the concentration of anhydrous calcium chloride was 0.2%, the concentration of glycerol was 0.5%, and the concentration of D-trehalose was 0.6%.

Embodiment 3

[0046] This embodiment provides a preparation method of a heparin detection reagent, the preparation method is roughly the same as the method provided in Examples 1 and 2, the difference lies in the different components, the difference is as follows:

[0047] The heparin detection reagent includes: heparinase I, anhydrous calcium chloride, glycerol and D-trehalose according to the mass percentage; wherein, the heparinase I is in a liquid state.

[0048] Specifically, the concentration of heparanase I was 7 IU / mL, the concentration of anhydrous calcium chloride was 0.3%, the concentration of glycerol was 0.4%, and the concentration of D-trehalose was 0.4%.

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Abstract

The invention, which relates to the technical field of anticoagulant effect detection, discloses a heparin detection kit and application thereof. To be specific, the heparin detection kit comprises aheparin detection reagent including heparinase I being a liquid enzyme preparation; for long-time preservation, the heparinase I needs to be frozen and dried and then the processed heparinase I becomes solid one for preservation; however, after a certain amount of anhydrous calcium chloride, glycerol and D-trehalose that are used as protective agents are added into the heparinase I, the detectionreagent exists stably for long time, so that the long-time preservation requirement is met. With the heparin detection kit, the anticoagulant activity of heparin can be neutralized rapidly and specifically without affecting other blood components of blood coagulation, so that the residual situation of the heparin sodium salt and the anticoagulant effect of heparin sodium salt can be evaluated rapidly and precisely and thus the basis is provided for clinical medication.

Description

technical field [0001] The invention relates to the technical field of anticoagulant effect detection, in particular to a heparin detection kit and its application. Background technique [0002] Heparin sodium salt is an anticoagulant, and it is the drug of choice for rapid anticoagulation. At present, it can be used clinically for surgical prevention of thrombosis and anticoagulant therapy for pregnant women. [0003] For patients with acute myocardial infarction, heparin can be used to prevent venous embolism in patients, and can prevent arterial embolism in patients with large anterior wall transmural myocardial infarction. It is used to treat disseminated intravascular coagulation (DIC) caused by various reasons, and is also used to treat glomerulonephritis, nephrotic syndrome, rheumatoid arthritis, etc. [0004] Currently, APTT (activated partial thrombin time) is mainly used to evaluate the anticoagulant effect of heparin clinically. The presence of heparin sodium sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
Inventor 张亚林黄蓓王华亮戎剑陈善钰张自彬陶光铖
Owner GUIZHOU JINJIU BIOTECH
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