Piercing neutral protease type time and temperature indicator

A neutral protease, time-temperature technology, applied in thermometers, thermometers based on physical/chemical changes, material analysis by observing the impact on chemical indicators, etc. Environmentally friendly, enhanced enzymatic activity, and low cost

Active Publication Date: 2019-05-03
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a phenomenon of "broken chain" in cold chain logistics, so consumers lack sufficient trust in the food of cold chain logistics. The development of a TTI that can visualize and truly reflect the shelf life of food can greatly enhance consumers' confidence in cold chain food

Method used

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  • Piercing neutral protease type time and temperature indicator
  • Piercing neutral protease type time and temperature indicator
  • Piercing neutral protease type time and temperature indicator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] According to the obtained parameters, four formulations of the neutral protease-type time-temperature indicator are obtained, as shown in Table 1, stored at 4°C, and the change trend of b* is observed and recorded, as shown in Table 1. figure 1 It can be seen that the change trend is regular and inversely proportional to time. The activation energies of the four formulations were measured separately. As shown in Table 2, the activation energies of the four TTI formulations were found to be between 51 and 63 kJ / mol, which can be used to judge the time under low-temperature transportation conditions. The neutral protease type time temperature indicator of formula 1 is carried out to observe the color change with time under different temperature conditions again, as figure 2 As shown, combined with the storage of grass carp surimi, the b* value was determined to be -38 as the end point, and the obtained change trend was consistent with the change trend of sensory evaluati...

Embodiment 2

[0044] A neutral protease-type time-temperature indicator system, the neutral protease-type time-temperature indicator system is composed of a color reagent, a substrate and an enzyme solution;

[0045] (1) The chromogenic reagent is 0.8 ml Folin phenol reagent, and the concentration is 2 mg / L;

[0046] (2) The substrate is a mixture of 9 ml of casein solution with a mass fraction of 2% and 1 ml of NaOH solution, wherein the casein solution is mixed with casein, 0.1 mol / L sodium hydroxide solution and distilled water;

[0047] (3) described enzyme solution is the neutral protease solution and 7 ml distilled water that 1.2 ml concentration is 2 mg / L;

[0048] (4) According to the above concentration ratio and adding order, add and mix sequentially, react at different temperatures, and observe the corresponding relationship between color change and reaction time.

[0049] Place the time temperature indicator obtained in Example 2 under different temperature gradients to observe t...

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Abstract

The invention relates to a piercing neutral protease type time and temperature indicator, which is consisted of a time / temperature indicator and a colourimetric card, the time / temperature indicator comprises a container comprising a chromogenic reagent, a substrate and enzyme; the chromogenic reagent is a Folin phenol reagent at a concentration of 2 mg / L; the substrate is a casein solution with mass fraction being 2%, which is composed of casein, 0.1 mol / L of a sodium hydroxide solution and distilled water; The enzyme is a neutral protease solution at the concentration of 2 mg / L. The inventionis a chromogenic system for an enzyme-substrate reaction designed according to a method for measuring protein concentration by Folin-phenol, irreversible color changes can be generated through time passing or temperature fluctuations, and a visible color change can be generated, which achieves the purpose of visually indicating the quality change of the frozen food after the time and temperaturechange.

Description

technical field [0001] The invention belongs to the technical field of cold chain logistics, and relates to a time-temperature indicator capable of monitoring the quality state and freshness of food in the process of cold chain logistics in real time. Background technique [0002] Fresh aquatic food requires a low temperature environment during storage and transportation, and fluctuations in storage temperature will have an important impact on the safety of this type of food. In order to ensure the safety of food, it is necessary to effectively monitor the storage temperature of food. In this way, the principle of least-shelf-life / first-out LSFO can be implemented based on the actual quality of the food. [0003] Time-temperature indicator (Time-Temperature Indicator, TTI) is an indicator that reflects the temperature change process experienced by food through the cumulative effect of time and temperature, usually manifested as mechanical deformation or color change, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/37G01N21/78G01K11/12
Inventor 谢晶黄文博王金锋
Owner SHANGHAI OCEAN UNIV
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