Pseudomonas plecoglossicida fliA gene silenced strain

Abstract

The invention provides a Pseudomonas plecoglossicida fliA gene silenced strain that is Pseudomonas plecoglossicida fliA-RNAi and deposited in the China Center for Type Culture Collection on November 12th, 2018 with an accession number of CCTCC NO:M 2018770. The constructed Pseudomonas plecoglossicida fliA gene silenced strain has virulence significantly lower than that of a Pseudomonas plecoglossicida wild strain, time of death caused by same infection conditions is delayed, and the death rate is greatly reduced, thus laying a theoretical foundation for development of an attenuated Pseudomonasplecoglossicida vaccine.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a strain of Pseudomonas mutans wxya Gene silencing strains. Background technique [0002] Pseudomonas mutans ( Pseudomonas plecoglossicida ) is the pathogenic bacterium of "visceral white spot disease" of mariculture fish such as large yellow croaker and oblique grouper, and the direct economic loss caused by it exceeds 100 million yuan every year. [0003] wxya The gene is a key gene in the synthesis of flagella, and its encoded FliA (σ 28 ) protein is essential in flagellar synthesis. σ 28 It is a positive regulatory protein in flagella synthesis, which determines the transcription of the third flagellar promoter, which controls the transcription of flagellar late genes, which encode flagellar motor proteins, trend proteins and flagellar silk proteins . When bacteria are absent wxya After the gene, its movement ability, membrane formation ability...

AI Technical Summary

Problems solved by technology

Pseudomonas mutans was discovered through large yellow croaker infection experiment wxya Gene silencing mutants cause delayed onset of death and greatly reduced mortality

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