Ruegeria mobilis strain and application thereof

A technology of Lugeria and movement, applied in applications, bacteria, animal feed, etc., can solve the problems of losing micro-ecological regulation, beneficial bacteria cannot be integrated into the target environment, etc., to reduce toxicity, suppress luminous intensity, and have no toxic side effects Effect

Active Publication Date: 2016-07-20
TONGWEI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the beneficial bacteria used in the past came from land or other water environments. Due to the difference in the living environment and the microbial flora, some beneficial bacteria may not be able to integrate into the target environment, thus losing the effect of microecological regulation

Method used

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  • Ruegeria mobilis strain and application thereof
  • Ruegeria mobilis strain and application thereof
  • Ruegeria mobilis strain and application thereof

Examples

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Embodiment Construction

[0015] The strains of the present invention will be described in detail below with reference to the examples.

[0016] 1. Screening of R. mobilis YJ3 strain

[0017] In April 2015, the inventor selected 10 healthy larvae seedlings from Hainan Haiyi Aquatic Seedling Co., Ltd., ground them, spread them on a plate of marine bacterial medium 2216E, and cultured them at a constant temperature of 28°C for 48 hours. A number of single colonies were randomly picked and planted on a plate coated with Luminescent Vibrio, and a light yellow colony was found to have a bacteriostatic zone around it, and then the single colony was picked for subculture until a purified strain was obtained, numbered YJ3.

[0018] Genomic DNA of strain YJ3 was extracted, and its 16S rRNA gene sequence was amplified by using bacterial universal primers. After sequencing, it was confirmed that the strain YJ3 screened by the present invention was Rugeria mobilis.

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Abstract

The invention relates to a Ruegeria mobilis strain YJ3 and application thereof. The preservation number of the strain YJ3 is CGMCC No.12381. According to the strain, by generating antimicrobial proteins, density induction quenching enzymes and the like, aquaculture diseases are prevented and controlled, and multiple common pathogenic bacteria can be inhibited, More importantly, the strain YJ3 has a remarkable inhibition effect on the luminous intensity of luminous vibrio and is capable of reducing the infection of luminous vibrio to artemia. The strain YJ3 has no toxic or side effects on shrimp seeds of Penaeus vannamei Boone and the artemia and can be used as a feed additive or an aquaculture water body additive to be applied to the aquaculture.

Description

technical field [0001] The invention belongs to the technical field of beneficial bacteria screening, and in particular relates to a strain of Rudgeria mobilis and its application. Background technique [0002] Shrimp farming is one of the important marine aquaculture industries in my country, which has brought huge economic and social benefits to our country. However, with the intensive, large-scale and high-density production of prawn farming, disease problems have become increasingly prominent. Vibriosis is the most common and most harmful bacterial disease in shrimp farming, which has caused serious economic losses in many countries in the world. The pathogenic bacteria that can cause vibriosis in prawns mainly include Vibriovulnificus, V.alginolyticus, V.anguillarum, V.campbellii, Vibrio brilliant bacteria (V.splendidus), mermaid Vibrio (V.damsela), prawn killing Vibrio (V.penaeicida), parahaemolyticus (V.parahaemolyticus) and Harvey Vibrio (V.harveyi) and so on. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61P31/04A23K10/18C12R1/01
CPCA61K35/741C12N1/205C12R2001/01
Inventor 于明超张晓华郑艳芬李志涛汤开浩李文涛蔡秀磊徐涛
Owner TONGWEI
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