Poria cocos moisture preserving emulsion and preparation method thereof
The technology of moisturizing lotion and Poria is applied in pharmaceutical formulations, cosmetic formulations, cosmetic formulations, etc., and can solve the problems of large ethanol, many operation steps, consumption, etc., achieve mild reaction, overcome easy agglomeration, and reduce side reactions Effect
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Embodiment 1
[0033] Described carboxymethyl pachyrhin is prepared by following method:
[0034] S1. Cultivation of Poria cocos: aseptically inoculate Poria cocos hyphae in wort juice liquid medium, and cultivate at 28°C for 4 days;
[0035] S2. Preparation of Poria cocos polysaccharide: Ferment the cultured Poria cocos medium at 35°C for 2 days, heat to a slight boil, extract for 1 hour, filter, add 3 times the amount of mixed solvent of chloroform and n-butanol, the chloroform and n-butanol The mixed volume ratio is 4:1, shaken, centrifuged, add 3 times the amount of 95% ethanol, overnight, filter to obtain a precipitate, wash 2 times with ether to obtain pachyman;
[0036] S3. Preparation of Poria Cocos Polysaccharide: Add 10g of Poria cocos polysaccharide into 100mL0.5mol / L sodium periodate solution for selective oxidation, adjust the pH value to 3 with trifluoroacetic acid, moderate acid hydrolysis, acid hydrolysis time is 30min, filter , washing the solid with water to obtain Poria c...
Embodiment 2
[0040] Described carboxymethyl pachyrhin is prepared by following method:
[0041] S1. Cultivation of Poria cocos: aseptically inoculate Poria cocos hyphae in wort liquid culture medium, and cultivate at 28° C. for 5 days;
[0042] S2. Preparation of Poria cocos polysaccharide: Ferment the cultured Poria cocos medium at 35°C for 2 days, heat to a slight boil, extract for 2 hours, filter, add 5 times the volume of a mixed solvent of chloroform and n-butanol, the chloroform and n-butanol The mixed volume ratio is 6:1, shaken, centrifuged, add 3 times the amount of 95% ethanol, overnight, filter to obtain a precipitate, wash 3 times with ether to obtain pachymane;
[0043] S3. Preparation of Poria Cocos Polysaccharide: Add 10g of Poria cocos polysaccharide into 30mL 1.5mol / L sodium periodate solution for selective oxidation, adjust the pH value to 4 with trifluoroacetic acid, moderate acid hydrolysis, acid hydrolysis time is 1h, filter , washing the solid with water to obtain Po...
Embodiment 3
[0047] Described carboxymethyl pachyrhin is prepared by following method:
[0048] S1. Cultivation of Poria cocos: aseptically inoculate Poria cocos hyphae in wort juice liquid medium, and cultivate at 28°C for 4 days;
[0049] S2. Preparation of Poria cocos polysaccharide: Ferment the cultured Poria cocos medium at 35°C for 2 days, heat to a slight boil, extract for 2 hours, filter, add a mixed solvent of chloroform and n-butanol, the mixing volume ratio of chloroform and n-butanol 5:1, shake, centrifuge, add 3 times the amount of 95% ethanol, overnight, filter to get precipitate, wash 3 times with diethyl ether to obtain pachyrhin;
[0050] S3. Preparation of Poria Cocos Polysaccharide: Add 10g of Poria cocos polysaccharide into 50mL 1.0mol / L sodium periodate solution for selective oxidation, adjust the pH value to 3.6 with trifluoroacetic acid, moderate acid hydrolysis, acid hydrolysis time is 45min, filter , washing the solid with water to obtain Poria cocos polysaccharid...
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