A method for detecting a plurality of small-molecule water-soluble matters simultaneously in a milk powder product

Abstract

The invention provides a method for detecting a plurality of small-molecule water-soluble matters simultaneously in a milk powder product. The method comprises the following steps: a) detecting a milkpowder product sample through a quantitative nuclear magnetic resonance method, and obtaining a <1>H-NMR spectrum; b) determining attribution of quantitative signal peak of each small-molecule water-soluble component in the<1>H-NMR spectrum; and c) carrying out deconvolution processing on each quantitative signal peak, the attribution of which is determined, and calculating the content of each small-molecule water-soluble component. The method is better in accuracy, stability, reproducibility and recovery than a traditional qNMR method, and provides technical support for formula research anddevelopment, product production, quality control and safety test of infant milk powders in the future.

Description

technical field [0001] The technical solution of the present invention belongs to the field of food detection, specifically to the field of component detection of milk powder products, especially to the field of component detection of infant formula milk powder. Background technique [0002] Infant formula milk powder is an artificial food that uses cow's milk, goat's milk, etc. as raw materials, and appropriately adds nutrients so that the total composition can supply the growth and development of infants. In order to simulate breast milk to the greatest extent and meet the growth and development needs of infants, manufacturers will add nucleotides, organic acids, amino acids and other additives according to the formula design. It plays an important role in improving the intestinal flora and promoting the body's metabolism. Therefore, it is extremely important to monitor the quality of small molecule water-soluble substances in formula milk powder. [0003] At present, na...

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Problems solved by technology

[0003] At present, national standards mostly use methods such as high performance liquid chromatography and spectrophotometry to use standard products as references to detect one or a class of substances. The disadvantages are: (1) only for a few of a class of substances It is difficult to fully reflect the true quality of milk powder through the detection of chemical compounds: for example, nucleotides are absorbed and utilized in the body in the form of nucleosides, both breast milk and infant formula milk powder contain nucleosides and nucleotides, but the national standard Only 5 kinds of nucleotide detection methods are stipulated, lack of nucleoside detection methods, it is difficult to fully reflect the true value of nucleotide components in milk powder; (2) low detection efficiency: limited by the principle of instrument detection, etc. High-performance liquid chromatography can only detect certain types or several types of chemical components under the same spectrum, and it is difficult to realize the simultaneous detection of multiple substances; (3) detection relies on standard products as references, and sometimes false positives may occur result

[0008] Therefore, it can be seen that in the current prior art, the exploration of how to use detection methods to simultaneously perform rapid detection of multiple components of formula milk products with a very complex composition cannot be said to be sufficient

Furthermore, there is room for further expansion in the development of applicable objects of various existing detection technologies

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