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Biological extraction method for fibers in phloem raw materials in waste crops

A technology of biological extraction and crops, which is applied in the field of resource utilization of discarded crops, can solve problems such as difficult strains, and achieve the effects of improving economic benefits, broad application prospects, and good economic benefits

Inactive Publication Date: 2019-04-26
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The basis of the biological extraction method is to obtain a fully functional degumming enzyme system for the removal of banana and pineapple fibrous gums, including pectinase, xylanase, mannanase, laccase, etc., but to obtain bacteria that secrete these enzymes at the same time very difficult

Method used

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  • Biological extraction method for fibers in phloem raw materials in waste crops
  • Biological extraction method for fibers in phloem raw materials in waste crops

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A kind of biological extraction method of pineapple leaf of embodiment 1

[0032] Such as figure 1 As shown, soak the dried pineapple leaf fiber raw material prepared by mechanical scraping with tap water, the bath ratio is 1: 15-20, and the time is 20 minutes. Ultrasonic treatment was performed for 20 minutes under the condition of power of 200W and frequency of 80kHz, the solid and liquid were separated, and the ultrasonic filtrate and fiber raw materials were collected separately for future use.

[0033] Pick a ring of DCE-01 strains from the original slant lawn preserved by the project team to inoculate 5.0mL of improved broth culture solution, suspend well, and incubate at 34°C for 5.5h. Spread on nutrient agar plate after dilution, incubate at 34°C for 18 hours, and isolate a single colony. Select typical colonies and inoculate them into 5.0 mL of improved broth culture solution, and incubate at 34°C for 5 hours, which is the primary seed solution. Inoculate th...

Embodiment 2

[0036] Embodiment 2 A kind of biological extraction method of banana bast fiber

[0037] Soak the dry banana bast fiber raw material scraped by machinery with tap water, the bath ratio is 1:20, and the time is 25min. After treatment with ultrasonic wave for 30 minutes under the condition of power of 200W and frequency of 80kHz, the solid and liquid were separated, and the ultrasonic filtrate and banana bast fiber raw material were collected respectively for future use.

[0038]Pick a ring of DCE-01 strains from the original slant lawn preserved by the project team to inoculate 5.0mL of improved broth culture solution, suspend well, and incubate at 34°C for 5.5h. Spread on nutrient agar plate after dilution, incubate at 34°C for 18 hours, and isolate a single colony. Select typical colonies and inoculate them into 5.0 mL of improved broth culture solution, and incubate at 34°C for 5 hours to obtain the primary seed solution. Inoculate the test tube solution into a 100mL Erlen...

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Abstract

The invention discloses a biological extraction method for fibers in phloem raw materials in waste crops. The biological extraction method comprises the steps of soaking the phloem raw materials in the waste crops into water, carrying out solid-liquid separation, respectively connecting filtrate and fiber raw materials, adding mature fermentation liquid of DCE-01 bacteria into the filtrate so as to obtain DCE-01 bacterial suspension, soaking the fiber raw materials into the DCE-01 bacterial suspension so as to degum the fiber raw materials, carrying out refining by virtue of laccase / medium, cleaning, and dehydrating. By virtue of DCE-01 bacteria degumming and laccase / medium refining, colloids such as pectin, hemicelluloses and lignin in fiber raw materials such as banana phloem and pineapple leaves can be properly removed; the fibers in the pineapple leaves and the banana phloem are acquired by virtue of an ''efficient, energy-saving and environment-friendly'' processing manner, namelythe waste is recycled, so that the economic benefit is increased, meanwhile, the sources of textile natural fibers are expanded, the economic benefit of comprehensive utilization is good, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of resource utilization of discarded crops, in particular to a method for biologically extracting fibers from bast raw materials of discarded crops. Background technique [0002] my country is one of the main banana and pineapple producing countries in the world. There are tens of millions of tons of bananas and pineapples for people to eat every year. At the same time, almost the same amount of banana and pineapple by-products such as banana stems and pineapple leaves are produced. Most of these by-products They are all discarded as rubbish, which seriously pollutes the environment. Banana bast and pineapple leaves contain a lot of cellulose fibers. If they are developed and utilized, they can not only expand the source of natural fibers for textiles, but also protect the environment. [0003] The chemical composition of banana bast fiber and pineapple leaf fiber is similar to other hemp fibers, as shown in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01C1/00C12N1/20C12R1/01
CPCC12N1/20D01C1/00
Inventor 成莉凤彭源德段盛文冯湘沅郑科杨琦刘志远
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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