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Method for reducing carbon dioxide to produce methane by optically driving methane octococcus

A methanosarcina and methanogenic technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex process, poor product selectivity, high energy consumption, etc., achieve simple process, reduce process The effect of low cost and energy consumption

Active Publication Date: 2019-04-23
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to target traditional CO 2 In the process of reducing methanogenesis, there are disadvantages such as complex process, high energy consumption, and poor product selectivity, and a method for reducing carbon dioxide to produce methanogenesis by light-driven Methanosarcina is provided

Method used

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  • Method for reducing carbon dioxide to produce methane by optically driving methane octococcus
  • Method for reducing carbon dioxide to produce methane by optically driving methane octococcus
  • Method for reducing carbon dioxide to produce methane by optically driving methane octococcus

Examples

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Effect test

Embodiment 1

[0045] (1) Methanosarcina barkeri was inoculated into an acetic acid medium with a pH of 6.87 at a volume ratio of 20%, the medium was adjusted, and the culture was maintained at a constant temperature of 37°C;

[0046] (2) When the OD of Methanosarcina barkeri in step 1 600nm When the value is 0.25, under the condition of 120r / min, add substance (CdCl 2 ) to synthesize CdS in the bacterial liquid (the acetic acid medium contains S 2- ), make its final concentration be 1mmol / L, obtain Methanosarcina barkeri-CdS hybrid system;

[0047] (3) The Methanosarcina barkeri-CdS hybrid system was centrifuged at 5000 r / min for 10 min, the precipitate was transferred to a carbon-free medium, and cysteine ​​was added to make the final concentration 0.15wt%, and cultured in The top of the base is fed with CO 2 ;

[0048] (4) Under the constant temperature of 37 ℃, use LED purple light (1.0mW / cm 2 ) to illuminate the hybrid system of step 3 with CO 2 Reductive methane production.

[0...

Embodiment 2

[0053] (1) Methanosarcina barkeri was inoculated into an acetic acid medium with a pH of 6.87 at a volume ratio of 20%, the medium was adjusted, and the culture was maintained at a constant temperature of 37°C;

[0054] (2) When the OD of Methanosarcina barkeri in step 1 600nm When the value is 0.25, under the condition of 120r / min, add substances to synthesize CdS in the bacterial liquid, so that the final concentration is 1mmol / L, and construct the Methanosarcina barkeri-CdS hybrid system;

[0055] (3) The Methanosarcina barkeri-CdS hybrid system was centrifuged at 5000 r / min for 10 min, transferred to a carbon-free medium, and the sacrificial reagent cysteine ​​was added to make the final concentration 0.15wt%, and cultured in The top of the base is fed with CO 2 ;

[0056] (4) Under the constant temperature of 37 °C, using different light intensities of 0.5, 1.0, 2.0, 4.0, 8.0 mW / cm 2 , the hybrid system of step 3 was subjected to light CO 2 Reductive methane productio...

Embodiment 3

[0059] (1) Methanosarcina barkeri was inoculated into an acetic acid medium with a pH of 6.87 at a volume ratio of 20%, the medium was adjusted, and the culture was maintained at a constant temperature of 37°C;

[0060] (2) When the OD of Methanosarcina barkeri in step 1 600nm When the value is 0.25, under the condition of 120r / min, add substances to synthesize CdS in the bacterial liquid, so that the final concentration is 1mmol / L, and construct the Methanosarcina barkeri-CdS hybrid system;

[0061] (3) The Methanosarcina barkeri-CdS hybrid system was centrifuged at 5000r / min for 10min, transferred to a carbon-free medium, and the sacrificial reagent cysteine ​​was added to make the final concentrations of 0.05, 0.1, 0.15, 0.2wt%, and pass CO on the top of the medium 2 ;

[0062] (4) Under the constant temperature of 37 ℃, use LED purple light (1.0mW / cm 2 ) to illuminate the hybrid system of step 3 with CO 2 Reductive methane production.

[0063] The results show that th...

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Abstract

The invention discloses a method for reducing carbon dioxide to produce methane by light-driven methane octococcus, which comprises the following steps: a nano semiconductor is deposited on the surface of Methanosarcina, and CO2 is introduced; Methanosarcina reduces CO2 to produce methane by using photogenerated electrons under the condition of light. According to the method, selective CO2 reduction to generate methane can be achieved by taking rich and cheap light energy as a driving force, and the process cost is obviously reduced. The method overcomes the defects of complex process, large energy consumption, poor product selectivity and the like in the traditional CO2 reduction methane production process; the method has the advantages of simple process, low energy consumption and strongproduct selectivity, so that large-scale popularization and application can be carried out.

Description

technical field [0001] The invention relates to a method for light-driven Methanosarcina to reduce carbon dioxide to produce methane. Background technique [0002] Due to the increasing frequency of human economic activities and the rising energy consumption, the global CO 2 emissions are increasing. subject to CO 2 Under the influence of the greenhouse effect, the global temperature will increase, which will lead to a series of adverse consequences such as melting of glaciers in the North and South poles and frequent catastrophic climates, threatening human life and other aspects of the environment, and is a major livelihood and strategic issue. . through effective means to remove CO 2 Reverting to methane with economic value is the key to resolving the two contradictions of "environmental pollution" and "scarcity of resources". [0003] Chemical catalysis is the realization of CO 2 One of the common strategies for reductive methanogenesis. But due to CO 2 Molecules...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12R1/01
CPCC12P5/023Y02E50/30
Inventor 周顺桂叶捷俞景
Owner FUJIAN AGRI & FORESTRY UNIV
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