Primer for identifying recessive white feather genotype of Xianglu mountain chickens and application of primer

Abstract

The invention provides a primer for identifying recessive white feather genotype of Xianglu mountain chickens and an application of the primer. Two pairs of primers, i.e., three primers, are adopted through design; double PCR amplification are carried out on the TYR gene colored feather allele C and the white feather allele c, the amplified PCR product is electrophoresed on 1.2% agarose gel electrophoresis, and then the agarose gel is photographed by a gel-imaging system, and the obtained electrophoresis pattern result is subjected to genotype determination: if only a 169 bp band can be obtained, the allelotype of the chicken to be tested is cc-type recessive white feather homozygotic type; if only a 526 bp band can be obtained, the allelotype genotype of the test chicken is CC colored feather homozygotic type; and if both bands of 169 bp and 526 bp are obtained at the same time, the allelotype of the chicken to be tested is a Cc colored heterozygous type. The primer disclosed by the invention has the characteristic of rapidly and accurately identifying colored feather homozygotic type and recessive homozygotic type and heterozygosis individuals of the Xianglu mountain chickens.

Description

technical field [0001] The invention relates to the technical field of gene molecular identification in molecular genetics, and relates to a primer for identifying the recessive white feather genotype of Xianglu pheasant and its application. Background technique [0002] The Xianglu pheasant is a primitive chicken breed formed under the long-term closed natural conditions of the Xianglu Mountain after the Miao people in the Kaili area migrated from the Central Plains to the southwest. The main wing feathers of the hen have flaky black feathers, and the tail has black feathers. Most of the feathers are sunflower-shaped. The back feathers of the rooster are brownish red, and the tail feathers are black with green luster. In Xianglu pheasant breeding, about 2 / 1000 white-feathered chickens appeared in the population. It was found that the recessive white-feathered allele would cause white spots on the feathers of chickens, resulting in a large proportion of elimination in breedi...

AI Technical Summary

Problems solved by technology

[0004] The molecular mechanism of recessive white feather formation is: the formation of recessive white feather is due to the insertion of a 7.7kp endogenous leukemia virus ALV sequence in the fourth intron region of TYR (tyrosinase gene), resulting in TYR gene transcripts The transmembrane domain lacking the fifth exon in the

As a result, tyrosinase loses its normal functional activity, resulting in the inability to synthesize melanin, resulting in the formation of chicken white feathers

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